外周血单个核细胞PSA mRNA及蛋白表达与前列腺癌微转移关系的研究

Study on relationship between the expression of PSA mRNA and protein in the mononuclear cells of the peripheral blood and prostate cancer micrometastasis

目的观察前列腺癌患者外周血单个核细胞PSA mRNA及蛋白表达,探讨其与前列腺癌微转移的关系。方法取抗凝血,Ficoll离心分离单个核细胞,RT-PCR检测PSA mRNA表达,Western blot检测PSA蛋白表达。结果(1)血清PSA>40．0ng／ml者23例,两者均表达的19例,阳性符合率82．61％(19／23);血清PSA <20．0ng／ml者16例,两者均表达的5例,阳性符合率31．25％(5／16);(2)PSA mRNA及蛋白的阳性表达与临床分期、Gleason评分均呈正相关;(3)血清PSA<20．0ng／ml的16例患者随访18个月,1例PSA mRNA及蛋白均表达阳性者,于前列腺癌根治术后半年内死于癌转移。结论外周血单个核细胞PSA mRNA及蛋白的联合检测是判断前列腺癌微转移的有效手段之一,并可预示前列腺癌的病理分级和不良预后。

Objective To detect the prostate cancer patients＇s expression of the PSA mRNA and protein in mononuclear cell of the peripheral blood and discuss the relationship between positive expression of two parameters and prostate cancer micrometastasis. Methods 5 ml anticoagulant blood was collected in every individual. The expression of the PSA mRNA and protein in the mononuclear cell of the peripheral blood were detected by combining RT-PCR with Western blot. Results （1） There were 23 patients whose serum PSA are more than 40.0ng/ml, the number of positive expression of the two methods were 19, the positivity coincidence rate was 82.61%;there were 16 patients whose serum PSA are less than 20.0ng/ml, the number of positive expression of the two methods were 5, the positivity coincidence rate was 31.25 %. （2） The positive expression of PSA mRNA and protein was positive correlated with clinical stage, Gleason score respectively by using Pearson correlation analysis. （3） The patients whose serum PSA was less than 20.0ng/ml were followed-up 18 months, and there was 1 patient had died of prostate cancer metastasis within 6 months after radical prostatectomy（RP） among 5 patients whose PSA. mRNA were positive expression as well as protein. Conclusion Detection of combinational PSA mRNA and protein may be regard as one of the effective method to detect prostate cancer micrometastasis, which may provide predictive value of poor differentiation and prognosis of prostate.