摘要
探讨三七皂苷Rg1对组织型纤溶酶原激活物(tPA)和纤溶酶原激活物抑制物(PAI-1)活性的调节作用。运用发色底物方法测定三七皂苷Rg1在体外和静脉注射对家兔血浆纤溶酶原激活物(tPA)和血浆或血小板释放的纤溶酶原激活物抑制物(PAI-1)水平的影响。结果表明,三七皂苷Rg1在体外呈浓度依赖性明显抑制血浆PAI-1活性,同时提高血浆tPA活性;30和60 mg/kg的三七皂苷Rg1静脉注射显著抑制血浆PAI-1活性,提高血浆tPA活性,同时降低凝血酶激活的血小板所释放的PAI-1水平。本实验提示三七皂苷Rg1能抑制PAI-1活性,同时升高tPA活性可能是其抗血栓作用的分子机制之一。
The modulating activity of Panax notoginsenoside Rg1 on type 1 plasminogen activator inhibitor （PAI-1） and tissuetype plasminngen activator （tPA） was investigated. The activity of PAI-1 in rabbit plasma or platelet-released substances and tPA in rabbit plasma was assayed using chromogenic substrate, in vitro or in vivo. The results showed that Panax notog/nsenoside Rg1, in vitro, significantly inhibited PAI-1 activity in plasma while elevating plasma tPA activity, in a concentration-dependent manner. Intravenous injection of Panax notoginsenoside Rg1 at 30 and 60 mg/kg markedly suppressed PAI-1 level in plasma as well as platelet-released substances stimulated by thrombin, while increasing plasma tPA activity. It is suggested that Panax notog/nsenos/de Rg1 inhibited PAI-1 activity and increased tPA activity, and this property of Panax notoginsenoside Rg1 is advantageous to its antithrornbotic effect.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第4期566-568,623,共4页
Natural Product Research and Development
