期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

羧肽酶B研究进展 被引量：2

Aduances in Carboxypeptidase B
下载PDF
导出
摘要 羧肽酶B是重组胰岛素生产中的一个必需工具酶,同时在蛋白质测序等领域中也得到广泛应用。文章综述了羧肽酶B的结构、测活方法、性质、稳定性及储存条件、提取方法、基因工程方法生产羧肽酶B的研究及重组羧肽酶B的性质等。 Carboxypeptidase B is a necessary enzyme especially in the processing of recombinant insulin and is often widely used in the sequencing of proteins. In this article, the structure, method of activity deter ruination, characteristics, stability, the storage condition, the resolution ways, the production and properties of recombinant carboxypeptidase B were all summarized.
作者 李素霞 张晓彦 张映新 杨梓 袁勤生
机构地区 华东理工大学生物反应器工程国家重点实验室
出处 《药物生物技术》 CAS CSCD 2006年第4期302-305,共4页 Pharmaceutical Biotechnology
基金 上海市生物化学重点学科资助
关键词 羧肽酶B 重组羧肽酶B 性质 Carboxypeptidase B, Recombinant Carboxypeptidase B, characteristic
分类号 Q55 [生物学—生物化学]
  • 相关文献

参考文献18

  • 1Aviles FX and Vendrell J,Carboxypeptidase B.In:Hand book of Proteolytic Enzymes,1998,1333-1335.
  • 2Gmois-Ruth FX,Cmopanys V,Qian Y,et al.Coll structure of avian carboxypeptidase D domain Ⅱ:A prototype for the regulatory metallocarboxypeptidase subfamily[J].EMBO J,1999,18:5817-5826.
  • 3San Segundo,Martinez MC,Vilanova M,et al.The severed activation segment of poreine pancreatic procarboxypeptidase A is a powerful inhibitor of the active enzyme[J].Biochin Biophys Acta,1982,707:74-80.
  • 4Coll M,Guasch A,Aviles FX,et al.Three-dimensional structure of porcine procarboxypeptidase B:a structural basis of its inactivity[J].EMBOJ,1991,10:1-9.
  • 5Burgos FJ,Salva M.Villegas V,et al.Analysis of the activation process of porcine procarboxypeptidase B and deter mination of the sequenece of its activation segment[J].Biochemistry,1991,30:4082-4089.
  • 6Folk JE,Piez KA,Carroll WA,et al.Carboxypeptidase B,IV.Purification and charaterization of the porcine enzyme[J].J Biol Chem,1960,235(8):2272-2277.
  • 7Folk JE,Wolff EC,Schirmer EW,et al.The kinetics of carboxypeptidase B activity.Ⅲ.Effects of alcohol on the peptidase and esterase activities; kinetic models[J].J Biol Chem,1962,237(10):3105-3109.
  • 8Graeme B,Ryno JN,Koji M,et al.Ostrich (Struthio camelus) Carboxypeptidase B:Purification,Kinetic Properties and Characterization of the Pancreatic Enzyme[J].Int J Biochem,1996,28(5):521-529.
  • 9Hideki Kishimura,Kenji Hayashi.Isolation and characteristics of carboxypeptidase B from the pyloric ceca of the starfish Asterias amurensis[J].Comparative Biochemistry and Physiology Part B,2002,133:183-189.
  • 10周颖,杨桦,肖尚志,乔德水.羧肽酶B的稳定性考察[J].中国生化药物杂志,2002,23(4):185-186. 被引量：4

二级参考文献30

  • 1张龙翔 张庭芳 等.SDS－聚丙烯酰胺凝胶电泳法测定蛋白质的相对分子量.生化实验方法和技术[M].北京:高等教育出版社,1989.100-106.
  • 2张龙翔 张庭芳 等.双缩脲法，生化实验方法和技术[M].北京:高等教育出版社,1989.100-106.
  • 3马建农 潘士皓 等.胰酶中多种蛋白水解酶的分离和纯化[J].医药工业,1988,19(9):385-389.
  • 4Gu ZY,Su ZG,Jan-Christer J.Urea gradient size-exclusion chromatography echanced the lysozyme refolding. J of Chromatography A,2001,918(2) :311-318.
  • 5Folk JE, Gladner K, Piez A, et al. Carboxypeptidases B. Biol Chem, 1960,235(3):2272-2277.
  • 6Batas B,Chaudburi JB. Protein refolding at high concentration using sizeexclusion chromatography. Biotechnol Bioeng, 1996,50(1): 16-23.
  • 7Li SX,Zhang YJ.Clong and expression of a new rat procarboxypeptidase B gene in Eschierichia coli and purification of recombinationt carboxypeptidase B. Pro Pep Lett,2003, 10(6):581-590.
  • 8Sambrook J, Frisch E F, Maniatis T. Molecular Cloning a Laboratory Manual 2nd ed [M]. USA: Cold Spring Harbor Laboratory Press, 1989.
  • 9Bradford M M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Anal Biochem, 1976,72: 248-254.
  • 10Clauser E, Gardell S J, Craik C S, et al. Structural characterization of the rat carboxypeptidase A1 and B: Comparative analysis of the rat carboxypeptidase gene family [J]. J Biol Chem,1988,263:17 837-17 845.

共引文献9

同被引文献20

  • 1Skidgel RA, Erdos EG. Cellular carboxypeptidases[J]. Immunological Reviews, 1998,161(1):129.
  • 2Pezzilli R, Morselli-Labate AM, Barbieri AR et al. Clinical usefulness of the serum carboxypeptidase B activation peptide in acute pancreatitis[J]. Journal of Petrology, 2000, 1 (3):58.
  • 3Chen JQ, Zhang HT, Hu MH, et al. Production of human insulin in an E. coli system with Met-Lys-human proinsulin as the expressed precursor[J]. Biotechnology and Applied Biochemistry, 1995,55(1) : 5.
  • 4Tietze F, Gladner JA, Folk JE. Release of C-terminal S-(beta-aminoethyl) cysteine residues by earboxypeptidase-B[J]. Biochimica et Biophysica Acta , 1957,26 (3): 659.
  • 5Folk JE, Gladner JA. Carboxypeptidase B. Ⅰ. Purification of the zymogen and specificity of the enzyme[J]. Journal of Biological Chemistry, 1958,231 : 379.
  • 6Folk JE, Gladner JA. Carboxypeptidase B Ⅱ. Mode of action on protein substrates and its application of carboxyl terminal group analysis[J]. Journal of Biological Chemistry, 1958, 231 : 393.
  • 7Li S X, Zhang Y J, Tian L P, et al. Cloning, Expression of a new rat procarboxypeptidase B gene in Escherichia coli and purification of recombination carboxypeptidase B[J]. Protein and Peptide Letters, 2003,10(6) : 1.
  • 8Salvador V, Virtudes V, Jane S, et al. Mapping the Pro-region of carboxypeptidase B by protein engineering, cloning, overexpression, and mutagenesis of the porcine proenzyme [J]. Journal of Biological Chemistry, 1999,274:19925.
  • 9Eaton DL, Malloy BE, Tsai SP, et al. Isolation, molecular cloning, and partial characterization of a novel earboxypepti dase B from human plasma[J]. Journal of Biological Chemistry, 1991,266: 21833.
  • 10Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72 : 248.

引证文献2

二级引证文献4

药物生物技术
2006年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部