摘要
目的探讨叶酸代谢中的亚甲基四氢叶酸还原酶(MTHFR)基因单核苷酸多肽与直肠癌风险的关系。方法以聚合酶链反应和限制性片段长度多态方法,对56例直肠癌患者和143例正常人对照的MTHFR基因C677T和A1298C基因型进行检测分析其对直肠癌风险的相关性。结果在正常对照组中,MTHFRC677TCC、CT、TT基因型频率分别为62·90%、35·08%和2·12%,而在直肠癌病人中分别为58·93%、26·77%和14·26%。A1298C与A1298C基因型在病例和对照中的分布差异无显著性意义。MTHFRA1298C基因型在病例和对照中的分布差异无显著性意义,1298变异基因型与677变异基因型之间无协同作用。结论MTHFR单核苷酸多肽不是中国人直肠癌的遗传易感性因素。
Objective To explore the relationship between methylenetetrahydrofolate reductase （MTHFR） polymorphisms and colorectal cancer. Methods Polymerise chain reaction and restriction fragment length polymorphism were used to analyze the polymorphisms of MTHFR genes C6677T and A1298C in 56 patients of colorectal cancer and 143 age-and-sex-matched control subjects. Results The distribution of MTHFR C677T and A1298C genotype was not significantly different between the two groups. Conclusion Single nucleotide polymorphism in the MTHFR gene is not a risk factor of colorectal cancer. Showing that no a joint effect exists between the MTHFR677 and 1298 polymorphisms on the risk of colorectal cancer.
出处
《现代医院》
2006年第9期4-7,共4页
Modern Hospitals
关键词
核苷酸类
直肠癌
易感性
基因
Nucleotides, colorectal cancer, Disease susceptibility, gene
