小鼠早期胚胎发育过程中细胞凋亡及凋亡基因表达的检测

Apoptosis and Expression of Apoptosis Genes during Mouse Early Embryonic Development

小鼠早期胚胎发育过程中凋亡现象大量存在,细胞凋亡与凋亡基因表达有关。应用彗星电泳法检测小鼠早期胚胎凋亡情况;应用巢式RT-PCR、免疫组化的方法检测了Bcl-2家族成员(Bax、Bcl-2、Bak、Bcl-xl)的表达变化情况。结果显示:随着胚胎细胞数目的增加,凋亡比率逐渐增大;Bax表达量在整个过程中基本不变,Bcl-2表达量逐渐上调,Bak、Bcl-xl的表达量逐渐降低。对小鼠早期胚胎发育过程中的基因表达研究对于揭示早期胚胎发育的机制有重大的意义。

There are a lot of apoptosis in early embryo of mouse, and it is induced by expression of apoptotic genes. In our experiment, we used Comet assay to observe the apoptosis during mouse early embryonic development, and nested RT-PCR and Immunocytochemistry to observe the expression of Bcl-2 family members （Bax, Bcl-2, Bak, Bcl-xl）. The result shows that the ratio of apoptosis gradually increase, while the cell number increase. In the whole process, Bax doesn＇t change much, the expression of Bcl-2 increase, while the expression of Bak, Bcl-xl decrease. The research on the expression of apoptotic genes during mouse early embryonic development has great significance to discover the mechanism of embryonic development.