转基因cry1Ab在高粱不同器官中的表达水平

Expression Level of Transgene cry1Ab in Different Organs of Sorghum

用Cry1Ab/Cry1Ac板式试剂盒测定了Cry1Ab蛋白在转基因高粱不同器官中的表达水平。结果表明,不同组织器官的Cry1Ab蛋白含量有明显差异,其顺序为叶鞘>叶>颖壳>籽粒>根>茎髓,在花药中则检测不到;不同部位的叶片、叶鞘和茎髓的Cry1Ab蛋白含量也有较大差异,中部叶的Cry1Ab蛋白含量高于上部叶和基部叶,叶鞘中是中部>基部>上部,茎髓中则表现为基部最高。

Expression level of crylAb gene in different organs of transgenic sorghum was detected by using Cry1Ab/CrylAc QuantiPlate Kits. There were obvious differences in CrylAb protein content in the different organs, with the highest in the leaf sheath, followed by the leaf, the glume, the seed, the root and the stem in order, while no CrylAb protein was detected in the anther. There were also obvious differences in Cry1Ab protein content in the leaf, the leaf sheath and the stem fragment at different positions. The CrylAb protein content in middle leaves was higher than that in top and base leaves, and higher Cry1Ab protein content was found in the middle leaf sheathes followed by the base and top leaf sheathes. However, the highest in the stem fragment was detected in the base.