丹芎抑凝饮对心脏瓣膜置换术后抗凝的实验研究

Experimental research of Danxiongyiningyin on anticoagulant of cardiac valve replacement

目的观察丹芎抑凝饮对家兔内源性、外源性凝血途径、优球蛋白溶解时间及血小板最大聚集率的影响。方法将家兔24只随机分为4组,即空白对照组、华法林组、丹芎抑凝饮高剂量组、丹芎抑凝饮低剂量组。各组按kg体重灌胃给药。定时抽血测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)和优球蛋白溶解时间、血小板最大聚集率。结果服药第5日丹芎抑凝饮低、高剂量组、华法林组与空白对照组比较PT、APTT均显著延长(P<0.05)。丹芎抑凝饮低、高剂量组与华法林组无统计学差异(P>0.05)。丹芎抑凝饮低、高剂量组优球蛋白的溶解时间(ELT)和血小板最大聚集率与空白对照组比较显著降低(P<0.01)。结论丹芎抑凝饮可使PT、APTT显著延长,对凝血过程中存在的内源性和外源性途径都存在干预作用。并能够显著抑制血小板的聚集,在某些方面可以替代华法林。

Objective To research influence of Danxiongyiningyin on endogenous, extrinsic coagulation and hemorheelogy in rabbit. Methods 24 rabbits were randomly divided into 4 groups. They were control group, Warfarin group, low-dose Danxiongyiningyin group and high-dose Danxiongyiningyin group. PT, APTT, whole blood viscosity, plasma viscosity, erythrocyte aggregation and erythrocyte deformability were observed in four groups. Results PT, APTT in low-dose Danxiongyiningyin group, high-dose Danxiongyiningyin group and Warfarin group were longer than those in control group 5 days after administration （ P〈0.05 ）. There were no significant differences between Warfarin group and low-dose Danxiongyiningyin group, high-dose Danxiongyiningyin group （ P〉0.05）. Improvement of hemorheology index in low-dose Danxiongyiningyin group, high-dose Danxiongyiningyin group was superior to those in Warfarin group （ P〈0.05）. The resuits were observed in whole blood viscosity, plasma viscosity, erythrocyte aggregation and erythrocyte deformability. Conclusion Danxiongyiningyin can prolong PT, APTT and influence endogenous, extrinsic coagulation. It can improve indexes of hemorheelogy.