细胞间黏附分子-1对卵巢癌单克隆细胞侵袭能力的影响

Effect of intercellular adhesion molecule-1 on invasion of ovarian monoclonal cancer cells

目的探讨细胞间黏附分子(ICAM)-1对卵巢癌单克隆细胞侵袭能力的影响。方法通过克隆技术和细胞电泳,从卵巢癌SKOV3细胞分离出具有高侵袭能力(S1)和低侵袭能力(S21)的单克隆细胞亚系,并接种于裸鼠体内。用免疫组化法观察裸鼠移植瘤组织中ICAM-1的表达。通过MTT法细胞黏附实验和Boyden小室法体外侵袭实验检测ICAM-1在S1细胞黏附侵袭过程中的作用。结果S1细胞株成瘤率高,瘤块与周围组织粘连明显;S1瘤块的ICAM-1表达高于S21瘤块。ICAM-1抗体对S1细胞黏附及侵袭人工基底膜能力的抑制率分别为41.9%和55.0%。结论ICAM-1在卵巢癌侵袭转移过程中发挥重要作用。

[Objective] To investigate the effect of intercellular adhesion molecule （ICAM）-1 on invasion of ovarian monoclonal cancer cells. [Methods] A high invasive subclone （S1） and a low invasive subclone （S21） were separated from human ovarian cancer line SKOV3 using cell clone technique and cell electrophoresis,and inoculated in nude rice. ICAM-1 expression in metastatic tissue of nude rice was detected by immunohistochemistry. The role of ICAM-I in adhesion and invasion of S1 cells was observed with cell adhesion test and Boyden Chamber invasive assay respectively. EResults] S1 cell mass showed higher adhesive capacity to surrounding tissue than S21 cell. ICAM-1 expression was significantly stronger in S1 than in S21. Positively stained cells were mainly located at the edge of the tumor. The antibody of ICAM-1 could significantly inhibit S1 cells from adhering to and invading the basement membrane matrix by 41.9% and 55.0% respectively. [Conclusion] ICAM-1 plays an important role in the adhesion and invasion of ovarian cancer.