异丙酚对内毒素诱导肺损伤MMP-2和MMP-9的影响

Effect of Propofol on MMP-2,MMP-9 in Lung Injury Induced by lipopolysaccharide (LPS) in Rats

目的:研究异丙酚对急性肺损伤时基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的影响。方法:健康SD大鼠30只随机分为3组:对照组经股静脉注射生理盐水;内毒素组经股静脉注射内毒素5 mg／kg后泵注生理盐水;异丙酚组经股静脉注射内毒素5 mg／kg后泵注异丙酚10 mg.kg1.h1。采取经股静脉注射内毒素复制肺损伤模型(5 mg/ kg)。4 h后放血处死动物,对气管肺泡灌洗液(BALF)行白细胞计数及分类,ELISA法检测血清和BALF中的MMP-2、MMP-9的活性。结果:内毒素组白细胞总数、巨噬细胞、中性粒细胞和淋巴细胞均明显高于其他两组(P<0．01)。异丙酚组白细胞总数明显高于对照组(P<0．01),巨噬细胞、中性粒细胞和淋巴细胞高于对照组(P<0．05);内毒素组血清和BALF中的MMP-2、MMP-9均明显高于其他两组(P<0．01),异丙酚组血清的MMP-2高于对照组(P<0．05)。结论:异丙酚可产生与抑制MMP-2、MMP-9活性相关的肺保护作用。

Objective：To study the effect of propofol on MMP-2,MMP-9 in acute lung injury in rats. Methods：Thirty SD rats were randomized into three groups each consisting ten rats：Control group,LPS group and Propofol group. The animal models were made by injected LPS via femoral vein（5mg/kg）. The leucocyte were accounted and classificated in BALF. MMP2, MMP-9 in blood serum and BALF were detected by ELISA method. Results： The number of leucocyte, macrophage, neutrophil, lymphocytes was significantly higher in LPS group than in other two groups（P〈0.01, respectively）. Compared Propofol group with the Control group,the leucocyte was significantly higher（ P〈0. 01 ） ,and the rest was higher（P〈0.05）. . The MMP- 2,MMP-9 in blood serum and BALF in LPS group were significantly higher than those in other two groups（P〈0.01）, respectively） ,and the MMP-2 in blood serum was higher in Propofol group than in Control group. Conclusion：Propofol can protect lung injury associating with intaibition of MMP-2, MMP9 activities.