幼儿急疹病因学的研究

StudyontheEtiologyofExanthemaSubitum

为了探讨幼儿急疹致病病毒，采用病毒分离培养、电镜、血清学试验、聚合酶链反应（ＰＣＲ）对１１例幼儿急疹患儿进行了研究。结果从７例患儿体内分离出一种病毒，此病毒能感染人脐血单一核细胞，产生典型细胞病变。电镜下可见感染细胞中疱疹样病毒颗粒；血清学试验证明分离株与ＨＨＶ－６ＧＳ株间存在抗原一致性；ＰＣＲ结果表明该分离株ＨＨＶ－６特异性ＤＮＡ阳性。综合以上结果，可以认定该分离株即为ＨＨＶ－６。我们同时还对幼儿急疹患儿做该病毒双份血清抗体效价测定，结果发现其恢复期血清抗体效价比急性期升高４倍以上，并能中和该分离病毒的感染性。

Inordertoexplorethecausalvirusforexanthemasubitum(ES),11patientswithESwerestudiedbyvirusisolation,electronmicroscopy,serologicaltestandPCR.Onevirusstrainwasiso-latedfromsevenpatients.Theisolatecouldinfecthumanumbilicalcordbloodmononuclearcels,andthecytopathiceffectscharacterizedbylargebaloon-likecelswereobservedafterinfection.Electronmi-croscopyofinfectedcelsinultrathinsectionsshowedenvelopedvirusparticleswhichweremorphologi-calysimilartoherpesgroupvirus.Serologicaltestfurtherdemonstratedthattheisolateswereanti-genicalyrelatedtoHHV-6GSstrain.HHV-6specificDNAwasdetectedinaltheisolatesbyPCR.AltheresultsconfirmedthattheisolatedviruswasHHV-6.Inaddition,pairedseracolectedfromESpa-tientsduringacuteandconvalescentphasesweretestedfortheantibodyagainstnewviralisolates.Theantibodytitreofconvalescentphasewas4timeshigherthanthatoftheacutephase,andtheconvales-centseracouldneutralizetheinfectivityofthenewisolates.TheresultsconfirmedthatthenewisolatedHHV-6wasthecausalagentforES.