矮牵牛花色CHS-A基因启动子(Pchsa)的克隆及序列分析

Cloning and sequence analysis of the promoter of CHS-A gene about flower color(Pchsa) from Petunia Hybrida

根据国外报道,矮牵牛花色相关基因CHS-A的启动子Pchsa具有花期特异性启动子的活性,人工合成2个特异性引物并从矮牵牛基因组中经PCR分离出长约500 bp的DNA片段,经纯化后测序得到499 bp的目的片段。在NCBI中通过BLAST程序与序列号为S52984的PchsA比较,其同源性为95.73%。应用DNAMAN软件进行序列分析,结果表明该片段除含有启动子的保守序列TATA-box、CAAT-box、GC-box、G-box外,还含有花期特异表达的启动序列TACPyAT-box。这为该序列在花卉改良中的进一步运用奠定了基础,同时也证明不同品种间的PchsA存在差异。

According to the worldwide reported sequence of PchsA promoter of CHS-A gene related to flower color in Petunia Hybrida , two spoeificities primers were designed and synthesized. A fragment about 500 bp from Petunia hybrida genomic DNA was isolatett by PCR. DNA sequeneing of this fragment showed that the target promoter of 499 bp was obtained. Comparison between the sequences of the reported promoter and the isolated fragment showed that their homology is 95.73 % .The sequenee analysis using DNAMAN software indicated that the fragment includes not only the conservative .sequences that the common promoters have： TATA-box, CAAT-box, GC-box, G-box but also the non-conservative sequence such as TACPyAT-box which promote gene expression in the poried of blossnming. This work will be useful for flower genetic improvement in future, and certificated again that different varieties would have difference in PchsA.