期刊文献+

矮牵牛花色CHS-A基因启动子(Pchsa)的克隆及序列分析 被引量:4

Cloning and sequence analysis of the promoter of CHS-A gene about flower color(Pchsa) from Petunia Hybrida
下载PDF
导出
摘要 根据国外报道,矮牵牛花色相关基因CHS-A的启动子Pchsa具有花期特异性启动子的活性,人工合成2个特异性引物并从矮牵牛基因组中经PCR分离出长约500 bp的DNA片段,经纯化后测序得到499 bp的目的片段。在NCBI中通过BLAST程序与序列号为S52984的PchsA比较,其同源性为95.73%。应用DNAMAN软件进行序列分析,结果表明该片段除含有启动子的保守序列TATA-box、CAAT-box、GC-box、G-box外,还含有花期特异表达的启动序列TACPyAT-box。这为该序列在花卉改良中的进一步运用奠定了基础,同时也证明不同品种间的PchsA存在差异。 According to the worldwide reported sequence of PchsA promoter of CHS-A gene related to flower color in Petunia Hybrida , two spoeificities primers were designed and synthesized. A fragment about 500 bp from Petunia hybrida genomic DNA was isolatett by PCR. DNA sequeneing of this fragment showed that the target promoter of 499 bp was obtained. Comparison between the sequences of the reported promoter and the isolated fragment showed that their homology is 95.73 % .The sequenee analysis using DNAMAN software indicated that the fragment includes not only the conservative .sequences that the common promoters have: TATA-box, CAAT-box, GC-box, G-box but also the non-conservative sequence such as TACPyAT-box which promote gene expression in the poried of blossnming. This work will be useful for flower genetic improvement in future, and certificated again that different varieties would have difference in PchsA.
出处 《西南农业学报》 CSCD 2006年第4期676-678,共3页 Southwest China Journal of Agricultural Sciences
基金 云南省科技攻关项目(2001NG22)
关键词 矮牵牛 CHS-A基因启动子 克隆 序列分析 Petunia Hybrida promoter of CHS-A gene cloning sequence analysis
  • 相关文献

参考文献7

  • 1Faktor O,Kooter J M,Dixon R A,et al.Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants revrals sequence motifs essential for floral expression[J].PMB,1996,32:849-859.
  • 2Ingrid M van der Meer,cornelis E Spelt,Josph N M Mol,et al.Promoter analysis of the chalcone synthase(chsA)gene of Prtunia hybrida:a 67 bp promoter region directs flower specific expression[J].Plant Mol.boil.,1990(15):95-109.
  • 3洪亚辉,蒋泓,萧浪涛,黄璜,张文.碧冬茄花特异表达基因启动子PchsA的克隆与序列分析[J].湖南农业大学学报(自然科学版),2003,29(6):474-477. 被引量:7
  • 4赵云鹏,陈发棣,郭维明.观赏植物花色基因工程研究进展[J].植物学通报,2003,20(1):51-58. 被引量:84
  • 5Tanaka Y,S Tsuda,T Kusumi.Metabolic engineering tomodify flower color[J].Plant Cell Physiology,1998(11):1119-1126.
  • 6Davis K M,S J Bloor,G.B.Spiller,et al,Production of yellow color in flowers:redirection of flavonoid biosynthesis in Petunia[J].Plant J,1998(13):259-266.
  • 7Mol J.Cornish,E.Mason J,et al.Novel coloured flowers[J].Current opinion in Biotechnology,1999(10):198-201.

二级参考文献13

  • 1傅荣昭,马江生,曹光诚,李文彬,孙勇如.观赏植物色香形墓因工程研究进展──文献综述[J].园艺学报,1995,22(4):381-381. 被引量:63
  • 2邵莉,李毅,杨美珠,宋云,陈章良,萧师辉.查尔酮合酶基因对转基因植物花色和育性的影响[J].Acta Botanica Sinica,1996,38(7):517-524. 被引量:80
  • 3[1]Ingrid M van der Meer, Cornelis E Spelt, Josph N M Mol, et al. Promoter analysis of the chalcone synthase (chsA) gene of Petunia hybrida:a 67 bp promoter region directs flower specific expression[J]. Plant Mol Biol, 1990,15:95-109.
  • 4[2]Mitchell K A, Markharn K R, Boase M R. Pigment chemistry and colour of Pelargoniun flower [J]. Phytochemistry,1988,47 (3) :355-361.
  • 5[3]Tanaka Y,Tsuda,Kusumi T. Metabolic engineering to modify flower color [J]. Plant Cell Physiology, 1998,(11):1119-1126.
  • 6[4]Brenda W S. Flavonoid biosynthesis: A colorful model for genetics, biochemistry ,cell biology and biotechnology [J]. Plant Physiol, 2001,126: 485- 493.
  • 7[5]Donner H K,Robbins T P. Genetics and developmental control of anthocyanin biosynthesis[J]. Ann Rev Genet,1991,(25):173.
  • 8[6]Sablowski R W, Baulcombe D C. Beran M. Expression of a flower-specific Myb protein in leaf cells using a viral vector causes ectopic activation of a target promoter[J].Proc Natl Acad Sci USA, 1995, 92:6901-6905.
  • 9[7]Solano R, Nicto C, Avila J, et al. Dual DNA binding specificity of a petal epidermis specific MYB transcription factor(MYB. Ph3) from Petunia hybrida[J]. EMBO J, 1995, 14:1773-1784.
  • 10包满珠.植物花青素基因的克隆及应用——文献综述[J].园艺学报,1997,24(3):279-284. 被引量:70

共引文献89

同被引文献86

引证文献4

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部