摘要
目的探讨外周血中巨噬细胞移动抑制因子MIF浓度与胃癌病理特征之间的关系,同时验证外周血MIF浓度与胃癌组织MIF蛋白和mRNA之间的相关性。方法30例胃癌患者均为温州医学院附属第二医院手术并经病理活检证实,按照分化、分期、浸润深度、有无淋巴结转移分组,所有患者均在入院初检测外周血中MIF浓度,同时在手术后取胃癌标本检测MIFmR-NA和蛋白的变化,另取温州医学院附属第二医院经胃镜检查健康者10例。结果胃癌患者的MIFmRNA、MIF蛋白、MIF外周血中浓度较健康对照组明显增高(P<0.05),MIF浓度的高低与分化程度、浸润深度、淋巴结转移密切相关(P<0.05),MIF浓度≥8.96ng/ml患者生存率较MIF浓度<8.96ng/ml患者生存率明显下降(P<0.05),外周血MIF浓度与胃癌组织中MIFmR-NA、MIF蛋白具有明显相关性(P<0.05)。结论外周血MIF浓度与患者分化程度、浸润深度、淋巴结转移密切相关,且可以很好预测胃癌组织中MIF基因和蛋白的变化情况。
OBJECTIVE to investigate the relationship between the concentration of MIF and the patho-parameter in gastric carcinoma as well as the correlationship between the concentration of MIF in peripherial blood and the expression of mRNA and protein of MIF in carcinoma tissue. METHODS 30 GC patients who testified by pathology biopsy were divided according to differentiation, infiltration depth, lymphonode metastasis, The concentration of MIF in peripherial blood of all patients was detected by ELISA after hospitalization, MIFmRNA and protein blood of all patients was detected by ELISA after hospitalization, MIFmRna and protein of carcinoma tissue were detected by RT-PCR, WESTERN BLOT methods, 10 other health objects were recruited. RESLUTS the MIFmRNA, protein and the concentration of MIF in peripherial blood of gastric carcinoma patients show siginificant difference with health objects （P〈 0.05）, the concentration of MIF has obviously correlation with the differetiation, infiltration depth, lymphonode metastasis （P〈0.05）, those patilents whoses MIF≥ 8.96 ng/ml have lower live rate than those whose MIF〈8.96 ng/ml, the concetration of MIF in peripherial blood has obviously correlation with the MIFmRNA, MIF protein in carcinoma tissue. CONCLUSION the concentration of MIF has obviously correlation with the differentiation, infiltration depth, lymphonode metastasis, at the same time, the MIF in peripherial blood can predict the state of MIF gene and protein in carcinoma tissue.
出处
《中国初级卫生保健》
2006年第9期79-81,共3页
Chinese Primary Health Care
