摘要
为了解凋亡对慢性乙型肝炎病变的意义，对３８例不同重度的慢性乙型肝炎病人的活检肝组织，以地高辛素标记的核苷酸（ＴＵＮＥＬ）试验原位检测肝细胞凋亡和以免疫组化检测Ｆａｓ表达。结果凋亡少见，而许多完好肝细胞核ＴＵＮＥＬ阳性，表示有ＤＮＡ损伤。轻型慢性肝炎１７例中，Ｆａｓ表达和ＤＮＡ损伤检测各有１４例阴性或弱阳性；中型７例无强阳性表达；重型１４例中Ｆａｓ表达强阳性９例，ＤＮＡ重度损伤３例。两者主要分布在碎屑样坏死区，与细胞坏死同时存在。
oelucidatetheroleofapoptosisinthepathologicallesionofhepatitisBvirus(HBV)infection,biopsiedlivertissuespecimensof38patientswithchronichepatitisBofvaryingseveritywereinvestigatedwithinsituimmunohistochemistryandTUNELtest.Apoptotichepatocyteswerefoundtoberare,whilethenucleiofmanycelswerepositivelystainedwiththeTUNEL,suggesting3′-OHendsgeneratedastheDNAwasimpaired.Ofthe17caseswithmildlesionorwithoutpiecemealnecrosis,14werenegativeorweaklypositivewithbothFasandTUNELtest.Ofthe7caseswithpiecemealandbri-dgingnecrosis,nonewasstronglypositive.Inthe14caseswithactivehepatitisandearlycirrhosis,stron-glypositiveresultswithFaswerefoundin9andwithTUNELin3respectively.ItissuggestedthatthecytotoxicTlymphocyte(CTL)-Fas-apoptosismechanismwasinvolvedinthehepatocytedeathofhepatitisBaswel.TheFasexpression,DNAdamageandapoptoticcelsdistributedmostlyinthepiecemealnecrosisregion,andthebalooningandthenecrotichepatocyteswerealsoclusteringinthisregion.AsboththeapoptosisandnecrosisaremediatedbyCTL,theyarecloselyrelated;whiletrans-ductedbydifferantways,theyoccurredindependently.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1996年第11期750-752,共3页
Chinese Journal of Internal Medicine
