采用RT-PCR技术检测甜樱桃“红灯”上的PNRSV

Detection of PNRSV in sweet cherry varity HongDeng' by RT-PCR

[目的]调查大连市特色水果品种甜樱桃“红灯”的田间植株携带PNRSV的情况．[方法]利用作者已建立的PNRSV RT-PCR检测体系．[结果]被检植株PNRSV的感染率达100％,但程度不同．[结论]对于其中感染PNRSV较轻的植株可以作为今后培育无PNRSV苗木的原材料．

[ Objective] To investigate whether the sweet cherry varity＂ gDeng＂ in hard was infected by PNRSV or not. [ Methods] The RT-PCR detection system published for PNRSV was used. [ Results] All the trees detected were infected by PNRSV,but the infected degree was different. [ Conclusion] The trees which were not infected seriously will be chosen as the primary materials to culture the young plants with PNRSV-free.