产肠毒素大肠杆菌k88ac基因原核融合表达载体的构建

Construction of Prokaryotic Fusion Expression Vector of ETEC K88ac Gene

利用PCR技术扩增出菌毛K88ac基因片段,并将其插入到原核表达载体pET32a(+)的多克隆位点上。测序结果表明:与Genbank中序列比对同源性达到99%以上,成功构建了原核表达载体。为进一步的研究工作,奠定了基础。

The gene K88ac was cloned by PCR, and was inserted into the multiple cloning sites of plasmid pET32a（＋）.The result of sequencing showed its homology was up to more than 99% compared with the sequence in Genbank, we successfully constructed the recombinant plasmid in prokaryocytic cell, and which provided the base for the further research work.