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体内TIMP-3基因电转染治疗人前列腺癌裸鼠移植瘤的研究

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摘要 目的研究肌肉内基因电转染对前列腺肿瘤的抑制作用。方法人前列腺癌细胞系PC-3M裸鼠皮下移植成瘤,TIMP-3质粒扩增后,通过电子穿孔仪(脉冲时值20 m s,电场强度200 V/cm)将质粒(15μg)转入裸鼠胫前肌。结果肌肉内TIMP-3电转染后,肿瘤生长受到抑制,较单纯TIMP-3肌肉内注射和空质粒(PCDNA-3质粒)肌肉内电转染有显著差异。结论肌肉内基因电转染,对前列腺癌有全身抗肿瘤作用。
作者 王金国 傅耀文 孙辉 单纯玉 周洪澜 常喜华 何尔斯泰
机构地区 吉林大学第一医院器官移植中心 吉林大学中日联谊医院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2006年第8期1073-1074,共2页 Chinese Journal of Gerontology
基金 吉林省科技发展项目(20030427-01)资助
关键词 电穿孔 前列腺癌 基因治疗
分类号 R737.25 [医药卫生—肿瘤]
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参考文献7

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二级参考文献15

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共引文献1

中国老年学杂志
2006年 第8期

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