白三烯B_4放射受体结合测定方法的建立及其特性的分析

STUDIES ON THE CHARACTERISTICS OF LEUKOTRIENEB4 RECEPTOR WITH RADIO-LIGAND BINDING ASSAY

白三烯Ｂ＿４放射受体结合测定方法的建立及其特性的分析赵宁，朱秀媛，程桂芳（中国医学科学院，中国协和医科大学药物研究所，北京１０００５０）白三烯Ｂ４（ｌｅｕｋｏｔｒｉｅｎｅＢ４，ＬＴＢ４）是花生四烯酸（ａｒａｃｈｉｄｏｎｉｃａｃｉｄ）５－脂氧酶代谢产物...

Leukotriene B4(LTB4),one of the metabolites of arachidonic acid via5-lipoxygenase (5-LO),plays important role in some inflammatory diseases as one of the most potentchemotaxis factor. A radio-ligand binding assay was set up and the characteristics of LTB4 receptor onguineapig splenocytes membrane were studied.At 25℃,the Kd was found to be 1.55 × 100-9mol·L-1 and the Bmax was 2.59×10-13 mol·mg-1 protein. The assay established was evaluated bynordihydroguaiaretic acid (NDGA) as positive control.