摘要
为观察给予庆大霉素后豚鼠在体椭圆囊壶腹嵴感觉细胞损伤后的恢复情况，实验组动物２５只连续１０～１４天注射１２５ｍｇ／ｋｇ庆大霉素。给药后的第１、３、７、１４、２２天处死各组动物，分别于处死前６小时给予动物腹腔内注射１５０ｍｇ／ｋｇ的５′－溴－２－脱氧尿苷（Ｂｒｏｍｏｄｅｏｘｙｕｒｉｄｉｎｅ，ＢｒｄＵ）。取一侧内耳的壶腹进行连续半薄切片的观察，另一侧壶腹进行免疫细胞化学方法观察。结果显示：损伤后的豚鼠壶腹嵴上皮细胞可以进行有丝分裂。
InthisinvivostudyweinvestigatedtheproliferationofcristaampularisinguineapigsafterGentamicinintoxicationbyBrdUimmunohistochemicalobservationandsemi-thinsections.TheexperimentalanimalsweregivenasingleintraperitonialinjectionofBrdUatadoseof150mg/kgbodyweightbeforesubsequentlykiledat1,3,7,14and22daypost-treatment(PT).SupportingcelproliferationwasevidentbyBrdUimmunoreactivityinthechromatinmaterialofdividingcelsat7and14dayPT.Theresultsdemonstratedthatthecristaampularisofguineapigshadrepairedbysupportingcelmitosis.
出处
《中华耳鼻咽喉科杂志》
CSCD
1996年第6期341-343,共3页
Chinese Journal of Otorhinolaryngology
