HPLC法测定珍稀渭片三组分药物含量

HPLC Law Determination Rare and Precious Wei Piece Three Components Medicines Content

目的建立高效液相色谱法测定珍稀渭片中丙谷胺、西咪替丁、尿囊素三组分的含量。方法内标法线性关系以组分与内标物的浓度比和峰面积比进行回归,得直线回归方程Y(尿)=0.467X-0.021r=0.999、Y(西)=2.884X-0.005r=0.9993、Y(丙)=1.328X-0.048r=0.9998。结果;HPLC法将珍珠粉与组分分离,珍珠粉在此色谱条件下不出峰,使珍稀渭片中各主要定量组分得到了快速、准确的定量。

Objective Establishes in the highly effective liquid phase chromatography determination rare and precious Wei piece third Gu An, west the onamot for Ding, the allantoin three components content. Methods Internal standard lawLinear relations：Compares by the component and the internal standard density with the peak area ratio carries on the return,results in the straight line regression equation：Y （urine）=0. 467X-0. 021 r=0. 399,Y （west） =2. 884X-0. 005,r=0. 999 3,Y（Third）=1. 328X-0. 048,r=0. 999 8. Finally;PH L the C law separates the tapioca and the component,the tapioca does not leave the peak under this chromatograph condition, enable in the rare and precious Wei piece each main quota component fast to obtain,the accurate quota.