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宏基因组学在新型生物催化剂开发中的研究进展 被引量：3

Advance in Metagenomics on the Development of New Biocatalyst
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摘要 宏基因组学是基因工程发展的新方向,它为寻找和发现新的功能基因及生物催化剂提供了新的研究策略。着重论述了宏基因组学的研究方法,包括DNA的提取、文库的构建以及筛选策略的选择。同时介绍了近年来宏基因组学应用于新型生物催化剂开发中所取得的一些成果。 Metagenomics is a new orientation of the development of genetic engineering. It provides a new strategy for seeking novel functional gene and biocatalyst. Methods in metagenomic study, including DNA extraction, library construction and screening were discussed in this paper. Meanwhile, the achievements of the application of metagenomics in the development of biocatalyst were also introduced.
作者 钱莉莉 陈少欣 史炳照
机构地区 上海医药工业研究院
出处 《微生物学杂志》 CAS CSCD 2006年第4期68-71,共4页 Journal of Microbiology
关键词 宏基因组学 不可培养微生物 生物催化剂 筛选 metagenomics uncultured microorganisms biocatalyst screening
分类号 Q93 [生物学—微生物学]
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参考文献22

  • 1Oh HJ,Cho KW,Jung IS.Expanding functional spaces of enzymes by utilizing whole genome treasure for library construction[J].J Mol Catal B:Enzymatic,2003,26(3):241-250.
  • 2Streit WR,Schmitz RA.Metagenomics-the key to the uncultured microbes[J].Curr Opin Biotechnol,2004,7(5):492-498.
  • 3张惠文,张倩茹,周启星,张成刚.分子微生物生态学及其研究进展[J].应用生态学报,2003,14(2):286-292. 被引量：40
  • 4Streit WR,Daniel R,Jaeger KE.Prospecting for biocatalysts and drugs in the genomes of non-cultured microorganisms[J].Curr Opin Biotechnol,2004,15(4):285-290.
  • 5Patrick Lorenz,Christa Schleper.Metagenome-a challenging source of enzyme discovery[J].J Mol Catal B:Enzymatic,2002,19(20):13-19.
  • 6Beja O.To BAC or not to BAC:marine ecogenomics[J].Curr Opin Biotechnol,2004,15(3):187-190.
  • 7Courtois S,Cappellano CM,Ball M,et al.Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products[J].Appl Environ Microbiol,2003,69(1):49-55.
  • 8Schloss PD,Handelsman J.Biotechnological prospects from metagenomics[J].Curr Opin Biotechnol,2003,14(3):303-310.
  • 9Tyson GW,Chapman J,Hugenholtz P,et al.Community structure and metabolism through reconstruction of microbial genomes from the environment[J].Nature,2004,428(2):37-43
  • 10Schmeisser C,Stockigt C,Raasch C,et al.Metagenome survey of biofilms in drinking-water networks[J].Appl Environ Microbiol,2003,69(12):7 298-7 309.

二级参考文献73

  • 1[1]Aharon O. 2002. Molecular ecology of extremely halophilic Archaea and Bacteria.FEMS Microbiol Ecol,39(1):1～7
  • 2[2]Alm DW, Zheng D, Raskin L.2000. The presence of humic substances and DNA in RNA extracts affects hybridization results.Appl Environ Microbiol,66:4547～4554
  • 3[3]Amann RI, Ludwig E,Schleifer KH.1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol Rev,59:143～169
  • 4[4]Andreas TP, Hosbond C, Nybtoe O. 2001. Identification of copper-induced genes in Pseudomonas fluorescens and use of a reporter strain to monitor bioavailable copper in soil.FEMS Microbiol Ecol,38(1):59～67
  • 5[5]Becker S, Boger P, Oehlmann. 2000. PCR bias in ecological analysis :A case study for quantitative Taq nuclease assays in analyses of microbial communities. Appl Environ Microbiol,66(11):4945～4953
  • 6[6]Bjrklf K, Jorgensen KS.2001. Applicability of non-antibiotic resistance marker genes in ecological studies of introduced bacteria in forest soil.FEMS Microbial Ecol,38(2-3):179～188
  • 7[7]Borneman J, Hartin RJ. 2000. PCR primers that amplify fungal rRNA genes from environmental samples.Appl Environ Microbiol,66(10):4356～4360
  • 8[8]Buchan A, Alber M, Hodson RE. 2001. Strain-specific differentiation of environmental Escherichia coli isolates via denaturing gradient gel electrophoresis (DGGE) analysis of the 16S-23S intergenic spacer region.FEMS Microbiol Ecol,35(3):313～321
  • 9[9]Buckley DH, Schmidt TM. 2001. Environmantal factors influencing the distribution of rRNA from verrucomicrobia in soil.FEMS Microbiol Ecol,35(1):105～112
  • 10[10]Burk T, Smmith H.1997.Editorial.Molecular Ecol,6:103

共引文献39

同被引文献130

引证文献3

二级引证文献18

微生物学杂志
2006年 第4期

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