摘要
我们建立了一种在酶标板上进行DNA:DNA杂交的方法,该方法与ELISA类似,简单、易操作、可半定量,便于临床实验室推广应用。对其杂交条件及影响因素进行了详细的探讨。通过该方法,地高辛标记的结核分支杆菌全染色体DNA探针和12种分支杆菌、7种非分支杆菌个染色体DNA的杂交百分率有显著差别,除卡介苗、堪萨斯分支杆菌杂交百分率超过30%,其余受试菌株均低于10%。结果表明在酶标板上进行核酸杂交是完全可行的,利用已知细菌DNA定量包被板,与未知的带标记的细菌DNA杂交,可达到菌种鉴定的目的。
We have developed a simple microplate hybridization method with a M.tuberculosis whole chromosomal DNA probe,and researched its conditions and influential factors of hybridization.This method is similar to ELISA,carried out easilier,and thus can be easily used in routine laboratories.By this method,hybridization percent of M.tuberculosis is notably different from that of the other mycobacteria and nonmycobacteria tested,except that of BCG and M.kansasii is higher than 30%,the others is lower than 10%.Hence.it is feasible to conduct the nucleic acid hybridization in the microplate.lf the microplate was coated with known mycobacteral DNA,and then hybridized with unknown labelled mycobacteral DNA,we will be able to conduct mycobacteral species identification.
出处
《中国防痨杂志》
CAS
1996年第4期177-180,共4页
Chinese Journal of Antituberculosis
关键词
结核杆菌
DNA探针
Bacteriologic technology M.tuberculosis