单宁酸-氯化铁法对SHR肾微血管密度的观察

Observation on Renal Microvessel Density by Tannic Acid-ferric Chloride Histochemistry

目的:探讨自发性高血压(SHR)病时肾内微血管密度的变化,及其与高血压发生的先、后关系。方法:用单宁酸-氯化铁组织化学方法媒染成年及幼年SHR、正常血压的雄性Wistar大鼠(WKY)4组大鼠肾毛细血管,并对血管密度进行定量分析。结果:与WKY成年组相比,SHR成年组肾皮质、肾髓质微血管密度均有所减低(P<0.05);血压尚未升高的SHR幼年组与WKY幼年组相比,肾皮质、髓质毛细血管密度差别无统计学意义(P>0.05)。结论:成年高血压大鼠存在肾微血管稀少的现象,因微血管稀少发生在高血压形成之后,推测它可能是高血压病引起的一种继发性改变;幼年高血压大鼠肾皮质微血管稀少的现象,提示微血管稀少也可能与遗传因素有关。

Objective： To explore the changes of renal microvessel density under hypertension and its relationship. Methods： Four group kidneys （n=10×4）ofaduh （ 12 m） and young （4 w） rats with spontaneously hypertension and Wistar-Kyoto rats with normal blood pressure were treated by tannic acid-ferric chloride histochemistry. The capillary densities of the renal cortex and the renal medulla were analyzed quantitatively by the image analysis system. Results： The microvessel density of renal cortex and medulla in adult rats with spontaneously hypertension were less than that in adult Wistar-Kyoto rats （P 〈 0.05）. There was no significant difference in microvessel density of renal cortex between young rats with spontaneously hypertension and the young Wistar-Kyoto rats （P 〉 0.05）. Conclusion： There is a microvessel rarefaction in the kidney of the adult rats with spontaneously hypertension. It may be a secondary change of hypertension. There is a microvessel rarefaction in the renal cortex of young rats with spontaneously hypertension. It may be related with heredity.