摘要
目的研究血红素加氧酶-1(HO-1)抑制剂锌卟啉(ZnPP)对自发性脑出血(ICH)大鼠脑水肿和远期脑萎缩情况的影响。方法应用立体定向仪固定大鼠,将自休新鲜动脉血100μL注入大鼠的右侧基底节区制作ICH实验动物模型,ICH大鼠分为ZnPP治疗组和DMSO溶剂对照组。部分大鼠在术后3 d断头取脑,采用干湿法测定脑组织含水量；部分大鼠在术后28 d灌注取脑作肜态学分析。结果ICH后3 d,ZnPP治疗组同侧基底节脑组织含水量明显低于DMSO对照组(P＜0．05)。ZnPP治疗组两侧基底节面积差值明显小于DMSO对照组(P＜0．05)。结论ICH后腹腔内应用HO-1抑制剂ZnPP缓释泵町以减轻ICH大鼠脑水肿程度和远期的脑萎缩。
Objective To investigate the inhibition of heme oxygenase-1 （HO1） inhibitor on brain edema and long term effects on brain atrophy after intracerebral hemorrhage （ICH） in rats. Methods 24 male Sprague Dawley rats received an intracerebral injection of 100 μL autologous blood. They were randomized into model group treated with Zinc Protoporphyrin （ZnPP） and DMSO control group. Parts of the rats＇ brain removed at day 3 after ICH were assessed for regional brain water content; parts of the rats brain removed at day 28 after ICH were performed computer assisted morphometric analyses. Results lay treatment with intraperitoneal ZnPP pump, occurrences of brain edema formation, and long term brain atrophy after ICH were significantly decreased （P〈0.05）. Conclusions Intraperitoneal ZnPP pump treatment is a new method of choice for managing ICH rats.
出处
《上海医学》
CAS
CSCD
北大核心
2006年第8期561-564,F0003,共5页
Shanghai Medical Journal
基金
国家自然科学基金(30400459)
关键词
血红素加氧酶-1
锌卟啉
自发性脑出血
脑水肿
Heme oxygenase-1
Zinc Protoporphyrin
Intracerebral hemorrhage
Brain edema
