摘要
为进一步探明三河马遗传分化,参考牛(M57764)、羊(AF002110)、猪(M17704)、人(J03071)和鼠(X12630)等哺乳动物GH基因序列,设计一对特异引物,用PCR方法从三河马基因组中扩增出一条长约2kb的DNA片段。PCR产物经pGEM-Teasy载体转化感受态DH5α株大肠杆菌,获得重组克隆子。DNA测序表明:三河马生长激素基因DNA序列长1923bp,含完整的5个外显子和4个内含子,与猪、狗、牛、羊的GH的cDNA序列同源性分别为94.47%,92.63%,90.06%和90.37%。其cDNA所编码氨基酸与猪、狗、牛、羊同源性分别为97.21%,96.74%,88.84%和88.37%,表明该基因在进化过程中是保守的。
Primers designed according to published literatures and the gene sequences of other mammals were used to amplify the full sequence of GH gene for the first time. The results showed , the full length of GH gene with 1923 bp consist of 5 exon, 4 intron. The comparison of these sequences with those of other animals through Blastn of GenBank, the results indicated that the identities of eDNA sequence of the GH gene were 94.47 %, 92.63 %, 90.06 % and 90.37 % by homologous comparison among Sanhe horse and other four species （pig, dog, cow and sheep）, and that were 97.21%, 96.74% ,88.84% and 88.37% in amino acid sequences.The results showed that the GH gene was conservative in the process of evolution.
出处
《华北农学报》
CSCD
北大核心
2006年第4期110-113,共4页
Acta Agriculturae Boreali-Sinica
基金
内蒙古自治区高等学校科学研究项目(NJ03093)
关键词
三河马
GH基因
克隆
序列分析
Sanhe horse
Growth hormone gene
Cloning
Sequence analysis
