预知子中α-常春藤皂苷的HPLC分析

Determination of Saponins P_(D) in Fructus Akebiae by High Performance Liquid Chromatography

目的分析不同种与不同产地预知子中α-常春藤皂苷（常春藤皂苷元-3-O-α—L-吡喃鼠李糖基（1→2）-α-L-吡喃阿拉伯糖苷）的含量，为预知子质量标准建立提供参考。方法采用高效液相色谱分析，Allteeh C18色谱柱，乙腈-水-磷酸（45：55：0．1）为流动相，检测波长203nm。结果口嫦春藤皂苷的保留时间为16min，线性范围为1．088—5.44μg （r=0.9999），加样回收率100．02％。所测样品中α-常春藤皂苷的含量在0.199—33．481mg·g^-1。结论该法快捷、简便、准确，可用于预知子的质量评价.

OBJECTIVE To establish a HPLC method for the analysis of saponins PD（hederagenin-3-O-α-L-rhamnopyranosyl（ 1→2）-α-L- arabinopyranoside） in different species and growing places. METHODS An Alheeh C18 column was used with aeetonitrile-water-phosphorie acid （45：55：0.1） as the mobile phase and UV detection was at 203 nm, RESULTS The relative retained time of saponins PD was 16 min, having a good recovery （100.02%） and linear correlation （ r = 0,999 9）, The contents of saponins PD were 0.199 - 33.481 mg· g^-1 in Fructus Akebiae from different growing places. CONCLUSION The method is simple and accurate, It can be used to control the quality of Fructus Akebiae.