抗原诱导下上皮内淋巴细胞形态学反应的实验研究

Experimental Studies on Morphologic Response of IEL by Antigen Induction

采用免疫组化和电镜方法，观察了６０只ＢＡＬＢ／ｃ小鼠经口伤寒杆菌诱导下上皮内淋巴细胞（ＩＥＬ）的形态、迁移和配布特征。结果表明，上述ＩＥＬ有Ｂ和Ｔ两种淋巴细胞，其中以Ｂ淋巴细胞为主。ＩＥＬ中Ｂ淋巴细胞有两种形态，一种细胞器较少，与上皮微绒毛之间有高电子密度，说明与抗原传递有关。另一种细胞器丰富，含大量扩张内质网，可能与蛋白分泌有关。因此提示ＩＥＬ不仅是粘膜免疫反应的效应场所，而且也是诱导场所。同时也提示了伤寒杆菌能诱导淋巴细胞向小肠上皮和ｄｏｍｅ上皮迁移。

In 60 BALB/c mice induced through oral typhoid bacteria,the morphologic,migratory and distributive characterization of IEL were observed by electron microscopy and immunohistochemistry.The results suggest that there are T and B two types lymphocytes in IEL,and B lymphocytes are mainly.There are two forms of these B cells in IEL.The first have a few organelles,there are some high electron dense substance between these cells and microvilli,these cells may be relative to antigen transfer.The second have plentiful organelles and expansible endoplasmic reticulum,these cells may be concerned with the production of antibody.Therefore,IEL is the mucosal immunity not only effector site but inductive site.These suggest that the typhoid bacteria may induce lymphocytes to migrate into the epithelia of intestine and dome area,and the epithelia of dome area accept antigen more easily than that of intestine.