两猪群间繁殖相关基因表达与生殖细胞数性状相关性研究(英文)

The Correlation of Reproduction-Related Gene Expression with Germ Cell Number in DM and PLL Gilts

采集三周龄杜洛克×梅山(DM,n=30)杂种母猪与PIC-长大(PLL,n=53)杂种母猪卵巢,测定其生殖细胞数目,比较2个猪群间生殖细胞数的差异,并对4个卵泡发育相关基因在2个猪群卵巢中的mRNA表达进行定量分析,研究了这些基因的表达与生殖细胞数之间的关系。结果表明:DM杂种猪生殖细胞数显著高于PLL杂种母猪(P<0.01);DM猪和PLL仔猪卵巢重没有明显差异(P=0.269),两猪群内生殖细胞数和卵巢重的相关性均不显著(分别R=0.335,P=0.07;R=0.119,P=0.398);DM杂种猪ESR和IGF1R的mRNA表达量与其生殖细胞数存在相关(分别R=0.648,P<0.05;R=0.757,P<0.01),FSHR和INHBAmRNA的表达与其生殖细胞数无显著相关;PLL母猪ESR、FSHR和IGF1RmRNA的表达与其生殖细胞数有相关性(分别R=0.435,P<0.01;R=0.438,P<0.01;R=0.292,P<0.05),INHBAmRNA的表达与其生殖细胞数无显著相关。

In this study, the ovarian germ cell number was counted in 3-week-old Duroc × Meishan （DM, n=30） and PIC × （Landrace × Large White） （PLL, n=53） gilts, and the mRNA expression levels of four reproduction-related genes were investigated by quantitative RT-PCR. Correlation of germ cell number with the expression level of these genes was analyzed. Results showed that the germ cell number of DM was significantly higher than that of PLL gilts （P〈0.01）, although there was no significant difference between the ovarian weight of DM and PLL gilts （P=0.269）. No significant correlation existed between germ cell number and ovarian weight in the two gilt groups （R=0.335, P=0.07; R=0.119, P=0.398, respectively）. A significant correlation was found between the germ cell number and expression level of ESR and IGFlR mRNA in DM gilts （R=0.648, P〈0.05; R=0.757, P〈0.01, respectively）, but the correlation between the germ cell number and expression level of FSHR and INHBA mRNA did not reach statistical significance. Significant correlation was found between the germ cell number and the expression level of ESR, FSHR, and IGFlR mRNA in PLL gilts （R=0.435, P〈0.01; R=0.438, P〈0.01; R=0.292, P〈0.05, respectively）, but not with INHBA mRNA in PLL gilts.