摘要
利用ISSR标记构建了24个选育桑品种的指纹图谱,用3种独立的方法(特殊的标记;特异的谱带类型;不同引物提供的谱带类型组合)可以有效地鉴别桑树选育品种,证明ISSR标记在桑树品种的鉴别方面是一个有效的工具和方法。17 个 ISSR 引物共扩增出 80 条带,40 条带具有多态性,占 50.0%。24 份选育桑树品种间平均遗传相似系数、Nei’s 基因多样性(gene diversity)和 Shannon’s 信息指数分别为0.8731,0.1210和0.1942。桑树选育品种间的遗传多样性较低,说明中国选育桑品种间遗传距离较小,亲缘关系较近,遗传基础较狭窄。UPGMA法聚类和PCA分析都清楚地显示了24个桑树选育品种的亲缘关系,聚类结果与桑树品种的系谱基本一致。
The ISSR fingerprintings of 24 mulberry cultivars were constructed. Totally 80 bands were produced using 17 primers selected from 20 primers. Of them, 40 bands showed polymorphism. From the bands amplified, there were three independent ways to identify the mulberry varieties, such as unique ISSR markers, unique band patterns and a combination of the band patterns provided by different primers. ISSRs were very effective in differentiating the mulberry varieties. The mean genetic similarity coefficient, the mean Nei＇s gene diversity （h）, and the mean Shannon＇s Information index （I） of mulberry cultivars were 0.8731, 0.1210, and 0.1942, respectively. This suggests that the genetic diversity of mulberry cultivars was low and the genetic base was narrow. Both UPGMA cluster and PCA （Principal Coordinates Analysis） analysis showed clear genetic relationships among the 24 mulberry cultivars. The major clusters were related to known pedigree relationships.
基金
This research was supported by the 10th Five-Year Key Programs for Science and Technology Develoment of China (No. 2004BA502B-01-07), National Infrastructure and Condition Platform Programs for Science and Technology Development (No. 2004DKA30390-09) and Chinese National Programs for High Technology Research and Development (No. 2003AA241220-1-6).
