摘要
目的:研究人重组肿瘤坏死因子-α(rhTNF—α)对人小梁细胞(human trabecular meshwork cells,HTM)凋亡的影响,探讨其在原发性青光眼发病中的作用机制。方法:组织块法原代培养人小梁细胞,取第3~5代细胞用于实验。不同浓度的rhTNF—α与细胞共同孵育24h,采用Annexin—V联合PI双染流式细胞术,测定细胞凋亡率,结合荧光显微镜来观查凋亡细胞。结果:浓度低于0.01MU/L的肿瘤坏死因子-α作用24h对人小梁细胞凋亡无明显影响,浓度0.01MU/L及以上的肿瘤坏死因子-α作用24h可明显增加入小梁细胞凋亡率,凋亡率与肿瘤坏死因子-α浓度成正比。结论:肿瘤坏死因子-α可以诱导体外培养的人小梁细胞发生凋亡,可能参与了青光眼发病中小梁细胞的损害过程。
AIM: To investigate the effects of the human recombinant tumor necrosis factor- alpha (rhTNF-α) on the apoptosis of human trabecular meshwork (HTM) cell.
METHODS: Primary and passage culture of HTM cells were established in vitro. The 3-5 passages of HTM cells were incubated with different concentrations of rhTNF-α for 24 hours, the apoptosis of HTM cells were detected by the method of Annexin V-FITC and PI stain, and the apoptosis rates were determined by the flow cytometer. The fluorescence microscope was used to recorded the apoptosis cells, which were stained by Annexin V-FITC.
RESULTS: rhTNF-α at the concentration 0.01MU/L and above induced apoptosis of the HTM cells, the apoptosis rate were raised depending on the concentration; while under 0.01MU/L, rhTNF-α seems has no effects on the HTM cells.
CONCLUSION: rhTNF-α can induce apoptosis of the cultured HTM cells in vitro, rhTNF-α may play an important role in the progress of glaucoma by damaging the HTM cells.
出处
《国际眼科杂志》
CAS
2006年第4期790-792,共3页
International Eye Science
关键词
小梁细胞
凋亡
肿瘤坏死因子-Α
青光眼
trabecular meshwork cell, apoptosis
human recombinant tumor necrosis factor-alpha
glaucoma