广西毛南族人群3个短串联重复序列基因座遗传多态性

Polymorphisms of three short tandem repeats loci in Maonan nationality in Guangxi province

目的：调查广西毛南族人群3个短串联重复序列(STR)基因座CSF1PO、TPOX、TH01的遗传多态性,探讨广西毛南族人群的起源。方法：应用荧光标记STR基因扫描技术分析200名广西毛南族无关个体；计算等位基因频率、遗传距离,构建系统发生树。结果：3个STR位点共检出18种等位基因,其频率分布在0．0050～0．4525之间；平均杂合度为0．6850,平均多态信息总量为0．6408,累积个体识别力达0．9969,累积非父排除率达0．9282。广西毛南族与广西壮族、广西水族、海南黎族、云南傣族、云南布依族的遗传关系较近。结论：广西毛南族3个STR基因座属于高度多态性遗传标记,本研究结果佐证了广西毛南族可能起源于百越民族。

Objective： To investigate the polymorphisms of 3 short tandem repeats （STR） loci （CSF1PO, TPOX, TH01） in Maonan nationality in Guangxi province and to explore the origins of Maonan nationality. Methods： Totally 200 unrealated individuals were analyzed by STR genescanning marked with fluorescence. Allele frequency and genetic distance were calculated and phylogenetic tree was constructed. Results： There were 18 STR allele in 3 STR of Maonan nationality with its frequencies ranging from 0. 0050 to 0. 452 5. The average heterozygosity was 0. 685 0; polymorphism information content was 0. 640 8; the total discrimination power was 0. 996 9; the accumulative probability of paternity exclusion was 0. 928 2. The genetic relationship between Maonan nationality and Zhuang, Shui nationality in Guangxi, Li nationality in Hainan and Dai, Bouyei nationality in Yunnan was closer. Conclusion ： The data obtained have high polymorphisms. The results help to prove that Maonan nationality possibly originates from Baiyue minority.