应用正交设计法优化长柄双花木不定芽生长条件

Optimization for Shoot Growth of Disanthus cercidifolius var.longipes with Orthogonal Design

通过正交设计方法L9(34)研究了培养基中的植物生长调节剂6-BA、NAA、GA对长柄双花木(D isanthuscercidifolavar.longipesH.T.Chang)不定芽生长速率的影响。实验结果表明:最优水平组合为6-BA 0.5 m.gL-1+GA 1.0 m.gL-1。BA对实验结果的影响达到极显著水平(p≤0.01),该因素不同水平间差异达到极显著水平(p<0.01)。

By applying orthogonal designed experiment L9 （3^4） in tissue culture of Disanthus cercidifola var. longipes H. T. Chang, effects of 6-BA, NAA, GA on its shoot growth rate were studied in this papper. The results indicate that the best combination of the plant regulators for shoot growth was 6-BA 0.5 mg·L^-1＋ GA 1.0 mg·L^-1. Factor BA influenced the shoot growth significantly （p ≤〈0.01 ）, and differences among the three concentration levels were remarkable significant （ p 〈0.01 ）.