油菜硫苷组分含量及抑菌活性研究

Composition and Content of Glucosinolates in Rapeseed and in vitro Inhibition of Botoytis cinerea Persoon

对油菜不同器官硫苷的组分与含量进行分析,结果表明根系和茎中的硫苷总量显著高于叶片,根系中的硫苷以4甲-氧基-3吲-哚甲基硫苷和1-甲氧基-3吲-哚甲基硫苷为主,茎和叶中以2羟-基-3丁-烯基硫苷和苯乙基硫苷为主。油菜根系中的硫苷组分从冬前期到终花期基本保持稳定,硫苷各组分的含量在不同生育时期的变化基本一致,终花期含量最高,成熟期含量最低。19个油菜品种根系中硫苷组分与含量存在较大差异;以草莓灰霉菌为靶标物,研究油菜根系的挥发性水解产物和水溶性水解产物对土传病原真菌的抑制作用,发现不同油菜品种根系水解产物的抑菌活性与其硫苷组分与含量有关,草莓灰霉菌对丁烯基ITC非常敏感,吲哚类ITCs对草莓灰霉生长的抑制作用要低于芳香族ITCs。

The composition and content of glucosinolates in root, stem and leaf of rapeseed （ Brassica napus） were analyzed. The results shows that within root 4-methoxyglucobrassicin and neoglucobrassicin predominated, while in leaf and mot tissue progoitrin and gluconasturtin were the most abundant. From pre-winter stage to final flowering stage, the profile of glucosinolates in mot were constant, while the contents of glucosinolates were congruously variable, highest in final flowering stage, and lowest in mature stage. There were significant differences between the profile and concentration of glucosinolates in mot of 19 rape varieties, which may partially account for differences in inhibition of B. cinerea mycelium growth by hydrolytic compounds release by mot of different rape varieties. It can be presumed that B. cinerea is very susceptible to butenyl ITC, and the mycelium growth inhibition of indole ITC was less than that of aromotic ITC.