布地奈德对变应性鼻炎豚鼠鼻粘膜嗜酸性粒细胞及组胺的影响

Effect of budesonide on eosinophils and histamine in nasal mucosa of guinea pig with allergic rhinitis

目的:制备变应性鼻炎动物模型,评价布地奈德对变应性鼻炎嗜酸性粒细胞和组胺的影响。方法:将豚鼠随机分成自然对照组、变应性鼻炎组和布地奈德治疗组,采用甲苯-2,4-二异氰酸酯对豚鼠鼻腔局部致敏激发制备变应性鼻炎模型,通过观察动物临床症状、鼻粘膜病理切片和鼻粘膜组织中组胺含量等指标来评价药物的药效。结果:变应性鼻炎组鼻粘膜组织中嗜酸细胞浸润和组胺含量均显著高于自然对照组(P<0.01或P<0.05);经治疗后,动物的嗜酸细胞浸润和组织中组胺含量均明显地降低(P<0.01)。在临床症状指标上,变应性鼻炎组和药物治疗组评分均明显高于自然对照组(P<0.01),但变应性鼻炎组和药物治疗组间无显著差异(P>0.05)。结论:布地奈德能有效地降低变应性鼻炎豚鼠鼻粘膜组织中嗜酸细胞浸润和组胺的含量,但未能改善动物的鼻部临床症状,这可能与造模的方法有关。

AIM： To build an allergic rhinitis model to evaluate the effect of budesonide on the eosinophils and histamine in nasal mucosa in allergic rhinitis guinea pig. METHODS： 33 guinea pigs were randomly divided into three groups： natural controlled group, allergic rhinitis （AR） group and budesonide treatment group. Allergic rhinitis model in guinea pigs were built by using toluene-2, 4-disocyanate （ TDI ） nasal immunization and challenge. The indexes of clinical symptoms, pathomorphological diagnosis and content of histamine in mucosa were used to evaluate the potency of budesonide when used to treat allergic rhinitis. RESULTS ： The expression of eosinophils and the content of histamine in nasal mucosa in AR group both were significantly higher than that of in natural controlled group （respectively, P 〈 0.01 and P 〈 0.05 ）. After the treatment, the expression of eosinophils and content of histamine in guinea pigs nasal mucosa were significantly decreased （both P 〈 0.01 ） compared with the AR group. The point rating of clinical symptoms of guinea pig nasal in both AR group and treatment group were higher than that of in natural group （P 〈 0.01 ）. However, there was no difference between AR group and treatment group （P 〉 0.05 ）. CONCLUSION： Budesonide could effectively reduce the expression of eosinophils and content of histamine in nasal mucosa, but it is invalid in releasing the symptoms of AR in guinea pig. The reason of this phenomenon may be concerned with the method of model building.