固定化脂质体色谱研究二至丸及其组成药物的经肠吸收成分

Analysis of biologically active compounds in Erzhi Pills and its compositions by immobilized liposome chromatagraphy

目的:应用固定化脂质体色谱(ILC)分析中药复方二至丸及其组成药物的经肠吸收成分及质量控制。方法:分别观察不同溶剂提取时提取液在ILC色谱柱上的色谱保留与分离,同时对在ILC色谱柱上保留的槲皮素及芹菜素进行了定量分析。结果:从ILC色谱柱保留峰的数目和峰面积两方面观察,墨旱莲的75%乙醇提取优于水提取。二至丸75%乙醇提取液中槲皮素的含量为9.6μg.g-1,芹菜素的含量为2.7μg.g-1。墨旱莲提取液中槲皮素的含量为2.8μg.g-1,芹菜素的含量为3.8μg.g-1。女贞子提取液中槲皮素的含量为9.1μg.g-1,而芹菜素用ILC未检测到。结论:槲皮素和芹菜素是二至丸中主要的可在ILC色谱上保留的成分,其中槲皮素来源于两个组成药物女贞子和墨旱莲,芹菜素仅来源于墨旱莲。槲皮素和芹菜素可能是二至丸的经肠吸收活性成分。固定化脂质体色谱有望成为中药及其复方肠吸收成分的初筛选和定量评价方法之一。

AIM： To analyse the intestinal absorbable compounds in Erzhi Pills and its compositions by immobilized liposome chromatagraphy （ ILC ）. METHODS： The ILC column separation and retention of the extracts of Erzhi Pills, Fructus Ligustri Lucidi and Herba Ecliptae extracted with different solvents were observed. Also analysed were the contents of quercetin and apigenin in 75% ethanol extract of Erzhi Pills, Fructus Ligustri Lucidi and Herba Ecliptae. RESULTS： It was found that the 75 % ethanol extract was better than the water extract based on the number and peak areas of retention peaks. In the 75 % ethanol extract of Erzhi Pills, the content of quercetin was 9.6 μg·g^-1, and the content of the apigenin was 2.7 μg·g^-1. The content of quercetin and apigenin in the 75 % ethanol extract of Herba Ecliptae corresponded to 2.8 and 3.8 μg·g^-1 respectivelly. In the 75 % ethanol extract of Fructus Ligustri Lucidi, the content of quercetin was 9.1 μg·g^-1, but apigenin was not detected. CONCLUSION： Quercetin and apigenin are two main retained components of Erzhi Pills on ILC, the former is contributed by both Fructus Ligustri Lucidi and Herba Ecliptae, the latter only by Herba Ecliptae. Quercetin and apigenin were predicted to be intestinal absorbable components of Erzhi Pills with ILC. Immobilized liposome chromatagraphy can be used as a tool in screening possible bioactive ingredients and in quality control of traditional Chinese medicine （TCM）.