刺梨提取物CL1对胃癌SGC-7901细胞增殖和人脐血CD34^+造血干/祖细胞增殖分化能力的影响

Effects of Rosa roxburghii Tratt extract-CL1 on gastric carcinoma SGC-7901 cells growth and human umbilical cord CD34^+ cells proliferation and differentiation

目的:观察刺梨提取物CL1对胃癌SGC-7901细胞增殖和人脐血CD34+造血干/祖细胞(HSPC)增殖分化能力的影响。方法:采用MTT法测定活细胞OD值,计算CL1对胃癌SGC-7901细胞增殖抑制率。免疫磁珠分选人脐血CD34+造血干/祖细胞,细胞记数、双色直接免疫荧光标记法和流式细胞仪分析细胞增殖分化能力变化。结果:MTT检测法显示,0.01~10μmol.L-1的CL1处理,剂量依赖性和时间依赖性地抑制胃癌SGC-7901细胞生长。经14 d的培养,与细胞对照组相比,10、1μmol.L-1的CL1细胞数有下降趋势,但差异无显著性;表达粒系/单核巨噬系细胞表型CD15和CD11b的细胞百分数无显著差异。经14 d的培养,与培养0 d比较,CD15显著增高(P<0.05),CD11b有增高趋势,但无显著性差异。结论:CL1对胃癌SGC-7901细胞的体外生长具有抑制作用,但对人脐带血CD34+HSPC的增殖、和向粒细胞、单核巨噬细胞分化无明显影响,表明CL1在抗肿瘤作用剂量下,不会明显抑制造血干/祖细胞向粒-单细胞的增殖分化,提示CL1可能不会引起明显的造血抑制。

AIM： To investigate the effects of Rosa roxburghii Tratt extract-CL1 on proliferation of gastric careinoma SGC-7901 cells, and on proliferation and differentiation of human umbilical cord （ UCB ） CD34^＋ cells. METHODS： The OD value of living cells was measured by MTT test. UCB CD34^＋ cells were isolated by magnetie-aetivated cell sorting, and surface marker of cells was determined using flow eytometry. Cells were counted by trypan blue dye exclusion test. RESULTS： The inhibitory percentages of CL1 （0.01 - 10 μmol·L^-1 ） on growth of SGC-7901 cells were concentration-dependent and time-dependent from 12 to 96 h. After culturing for 14 d, compared with control group, there was a downtrend of the numbers of CL1 10 and 1 μmol·L^-1 but there was no signifieanee（P 〉 0.05）, and there were no difference in the cell number percentage of both expressing CD15 and expressing CDllb. But compared with d 0, the cell number percentage of expressing CD15 were remarkably increased ; the cell number percentage of expressing CD11b had an increasing tendency, but but it. was not significant （P 〉 0.05） CONCLUSION： CL1 has inhibitory effect on growth of SGC-7901 cells in vitro, but does not affect proliferation of CD34^＋ and differentiation of CD34^＋ haematopoietic stem/progenitor cells to granulocyte and monocytes. This demonstrates that CL1 has anticancer effect, but does not affect the proliferation and differentiation of haematopoietic stem/progenitor cell to granulocytes and monocytes. It may not cause obvious haematopoietic depression.