土贝母皂苷体外诱导SW480细胞凋亡作用

Mechanisms of apoptosis induced by tubeimosides in human rectal cancer cell line SW480 in vitro

目的探讨土贝母皂苷甲、丙对于SW 480肿瘤细胞的体外诱导细胞凋亡作用机制。方法通过光镜或电镜观察药物干预后SW 480细胞形态改变;采用流式细胞仪测定FITC-Annexin V/PI双标记后SW 480细胞凋亡早期信号;用免疫细胞化学的方法观察药物对细胞中Bc l-2,p53,Fas蛋白表达的影响。结果药物组与对照组比较,从细胞形态中观察到细胞凋亡的特征结构;用流式细胞仪检测到凋亡细胞和坏死细胞数量均有增加;免疫细胞化学检测表明土贝母皂苷是通过抑制Bc l-2,p53基因和上调Fas基因表达诱导细胞凋亡。结论土贝母皂苷甲、丙能够诱导SW 480细胞凋亡,这可能是土贝母皂苷抗肿瘤作用的途径之一。

Aim To study the effect of tubeimosides on human rectal cancer cell line SW480 in vitro and the mechanisms of its antitumor effect. Method The morphological changes were determined by means of light microscopy and electron microscopy respectively. Cell apoptosis was detected by flow cytometry through Annexin V assay and PI fluorescent staining methods. The protein levels of Bcl-2, p53 and Fas were determined using immunohistochemical technique. Results Apparent morphological characteristic of apoptosis was de-tected under the optical and electric microscope. The percentage of apoptotic cell increased when comparing the drug groups with control after treatment with tubeimoside Ⅰ and Ⅲ. Immunohistochemical analysis revealed apoptosis was and induced by tubeimosides through inhibiting Bcl-2 and p53, and upregulating Fas. Conclusion Tubeimosides can induce apoptosis of SW480 cells, which may be one reasons of its antitumor effects.