油松胚珠发育研究中的蛋白质组技术(英文)

Proteomic Technique Used in Studying Ovule Development of Chinese Pine (Pinus tabulaeformis Carr.)

油松是中国特有的树种,具有重要的造林和绿化价值。但是油松胚珠败育是油松种子生产和繁殖面临的一个严重的问题,因此研究油松胚珠的发育机理和败育原因具有重要的理论和实践意义。双向电泳和质谱技术结合研究基因表达的蛋白质组技术正越来越广泛地应用到植物研究中,我们将该技术应用到油松胚珠发育的研究中,比较了两种蛋白质提取的方法,发现蔗糖提取方法比传统的TCA 方法更适合于油松蛋白质的制备。将电泳分离得到的蛋白点用肽质量指纹图谱的方法进行鉴定,得到了满意的结果,优化了蛋白质质谱鉴定的条件。

Chinese pine （Pinus tabulaeformis Cart.） is one of the most important afforestation and ornamental species in China. Ovule abortion is a serious problem influencing the output of seeds and reproduction of Chinese pine. So it is of much significance to study the mechanisms of ovule development and abortion of Chinese pine. By combining two-dimensional gel electrophoresis （2-DE） and mass spectrometry analysis, the patterns of gene expression in a specific tissue at a specific stage can be displayed and characterized. This study acquired an appropriate protein extraction method from ovules of Chinese pine and optimized the conditions for protein identification by mass spectrometry.