3种神经肽在血管运动性鼻炎发病中的作用

The roles of three peptides in the pathogenesis of vasomotor rhinitis

目的:通过研究以P物质(SP)、降钙素基因相关肽(CGRP)和血管活性肠肽(VIP)为代表的神经肽及其神经纤维在血管运动性鼻炎患者鼻黏膜中的表达、分布,探讨其在血管运动性鼻炎发病中的可能作用。方法:实验组鼻黏膜标本来源于具有典型临床症状和体征,并经辅助检查证实的30例血管运动性鼻炎患者,根据是否接受过治疗,将实验组分为治疗组和未治疗组,每组15例;对照组鼻黏膜标本来源于近3个月无鼻腔急慢性炎性病变并排除变应性疾病的9例患者。采用免疫组织化学染色法和医学图像处理分析系统,定性、半定量分析并比较SP、CGRP、VIP在实验组和对照组鼻黏膜中的表达、分布间的差异。结果:实验组鼻黏膜中SP、CGRP、VIP阳性神经纤维和细胞的数目明显增多,而且其平均积分吸收度明显高于对照组并具有统计学意义(P<0.05),治疗组和未治疗组间的差异均无统计学意义(均P>0.05)。结论:以SP、CGRP及VIP为代表的神经肽及其神经纤维系统在血管运动性鼻炎发病中可能起到重要的作用。

To explore the possible roles of three neuropeptides in the pathogenesis of vasomotor rhinitis by studying the expression and distribution of neuropeptides in the mucosa of vasomotor rhinitis, such as Substance P（SP）, calcitonin gene related peptide （CGRP） and vasoactive intestinal peptide（VIP）. Method： The mucosa specimens of thirty vasomotor rhinitis patients who had typical symptoms and signs were selected randomly as the experiment group and were divided into two subgroups depending on if received treatment or not. While the normal middle turbinate mucosa specimens of nine cases were selected as the control group. The expression and distribution of neuropeptides were examined by immunohistochemical SP method and computer image disposing and analyzing system. Result： The terminals of SP, CGRP and VIP in the treated experiment group wet and untreated experiment group were markedly increased in density of immunostaining compared to the control group, and the difference is significant （ P〈0.01）. Conclusion ： Neuropeptides, such as SP, CGRP and VIP , may play important roles in the pathphysiological mechanism of vasomotor rhinitis.