人鼻咽癌CNE细胞摄取^(99m)TcN(NOEt)_2与^(99m)Tc-MIBI动力学对比研究

Comparative study of uptake and washout of ^(99m)TcN(NOEt)_2 with ^(99m)Tc-MIBI in human nasopharyngeal carcinoma cell line CNE

目的:研究99mTc-氮-二{(N-乙基-N-乙氧基二硫代氨基甲酸盐[N(NOEt)2]}和99mTc-甲氧基异丁基异腈(MIBI)在人鼻咽癌CNE细胞中的摄取动力学。方法:用放射性核素示踪技术研究人鼻咽癌CNE细胞在37℃和22℃时对99mTcN(NOEt)2和99mTc-MIBI的摄取动力学规律。结果:37℃和22℃时CNE细胞对99mTcN(NOEt)2的摄取差异无统计学意义,99mTc-MIBI摄取则呈温度依赖性。37℃时CNE细胞对99mTcN(NOEt)2摄取峰值为43.15%,99mTc-MIBI为11.08%(P<0.01);5min时CNE细胞摄取99mTcN(NOEt)2为峰值的63%,99mTc-MIBI为48%(P<0.05);60min时99mTcN(NOEt)2在CNE细胞中的滞留率为63.92%,99mTc-MIBI为53.97%(P<0.05)。结论:99mTcN(NOEt)2可能较99mTc-MIBI更适用于鼻咽癌显像,具有潜在的临床应用价值。

To determine the cellular kinetics of ^99mTe-bistN-ethoxy-N-ethyl dithiocarbamato- nitrido EN（NOEt）2] in human nasopharyngeal carcinoma CNE cells line and to compare it with the cellular kinetics of ^99mTc methoxyisobutylisonitrile （MIBI） in the same conditions; to define the possible clinical application value of ^99mTcN（NOEt）2 in nasopharyngeal carcinoma imaging. Method：^99mTcN（NOEt）2 or ^99mTc-MIBI were incubated with CNE cells at 37℃, 22℃, at different time points after incubation, the uptake and washout characteristics of radiotracers in CNE cells were investigated and compared. Result：The maximum uptake ratio of ^99mTcN（NOEt）2 in CNE cells was 43.15%, that of ^99mTc-MIBI was 11. 08%（ P〈0. 01）. The uptake of ^99mTcN（NOEt）2 after 5 min incubation in CNE cells was 63% of total uptake, uptake of ^99mTc-MIBI was 48% of total uptake（ P〈0. 05） ;1 h after removal of the unbound tracer, 63.92% of the total uptake of ^99mTcN（NOEt）2 was retained in the CNE cells, 53. 97% of ^99mTc MIBI was retained （ P〈0. 05）; not like ^99mTc-MIBI, the cellular kinetics of ^99mTcN （NOEt）2 were similar at 37℃ and 22℃ （ P〉0.05）. Conclusion：^99mTcN（NOEt）2 may be a gamma imaging agent better than ^99mTc-MIBI for nasopharyngeal carcinoma imaging. ^99mTcN（NOEt）2 is a potential human nasopharyngeal carcinoma imaging agent.