化疗对结肠癌Th1和Th2类细胞因子基因表达的影响及意义

The Influence and Significance of Chemotherapy for the Gene Expression of Th1 and Th2 Cytokines in Colon Carcinoma

目的:探讨结肠癌Th1和Th2类细胞因子的基因表达水平及化疗对其表达的影响。方法:采用RT-PCR技术,检测35例正常人、30例结肠息肉和67例结肠癌患者及结肠癌组化疗后外周血单个核细胞(PBMC)Th1和Th2类细胞因子mRNA表达水平。结果:正常对照组及结肠息肉组Th1和Th2类细胞因子基因表达呈阴性或基本不表达。结肠癌组IL-4、IL-6、IL-10mRNA表达水平显著高于正常对照组及结肠息肉组(P<0.05);IFN-!无阳性表达(0/67),IL-2则仅有3例阳性表达。化疗后Th1类细胞因子表达增强,Th2类细胞因子的表达则减弱(P<0.05)。结论:结肠癌患者呈Th2类细胞因子强势表达,细胞免疫受抑,化疗可纠正Th1/Th2的失衡状态,甚至使Th2类向Th1类漂移。

To observe the influence of chemotherapy on the switch between Thl and Th2 cytokines in colon carcinoma. Methods： The gene expression of Thl and Th2 cytokines was detected by reverse transcription polymerase chain reaction （RT-PCR） in 35 normal controls, 30 cases with benign colon polyps and 67 patients with colon carcinoma before and after chemotherapy. Results： Thl and Th2 cytokines were negative in peripheral blood from those with benign polyps and the normal controls. The mRNA expression of IL-4, IL-6, and IL-10 in PBMCs from patients with colon carcinoma was significantly higher than in patients with benign polyps or the normal controls （P〈0.05）. IFN-γ expression was not detected in any patients （0/67） before treatment, and the detection of IL-2 expression was only 3/67. After chemotherapy, the expression of Thl and Th2 cytokines in colon carcinoma was significantly changed. There was also a significant enhancement in IFN-γ and IL-2 expression paralleled by a substantial decrease in IL-4, IL-6, and IL-10. Conclusion： The expression of Thl cytokine was inhibited and there was a concomitant state of Th2 cytokine predominance in PBMCs from patients with colon carcinoma. Chemotherapy can convert the imbalanced state of Thl and Th2 cytokines present in colon carcinoma and make it incline more toward normal.