香烟和绿茶对辐射致突变效应的影响

Influence of cigarette smoke and green tea on radiation-induced micronucleated polychromatic erythrocytes

目的探讨日常生活中的香烟和绿茶因素对辐射致突变效应的影响,为放射损伤的防护提供基础资料。方法按照析因实验设计,香烟、绿茶和辐射3因素用与不用2个水平(23)分为8个实验组。动物分组采用分层随机化。各组分别给予香烟、绿茶和辐射,以及3因素的各种不同组合,其中1组为空白对照组。单盲法测定小鼠骨髓嗜多染红细胞微核率。实验数据用SAS8·0分析软件进行析因实验设计的方差分析。结果对于诱发骨髓细胞突变的效应,辐射因素、香烟因素、绿茶因素,其中香烟与辐射的交互作用差异有统计学意义(P<0·01),绿茶与辐射的交互作用差异有统计学意义(P<0·05)。结论辐射和吸烟均具有致突变作用,绿茶具有抑制细胞突变的作用,吸烟与辐射有协同致突变作用,绿茶对辐射的致突变性有拮抗作用。

Objective To observe the effects of cigarette smoke and green tea on radiation-induced bone marrow cell mutation, and to provide scientific information for prevention and treatment of radiation damage. Methods There were 8 groups in the factorial experiment design with 3 factors at 2 levels. Mice were randomly divided into each group. There were 8 mice in each group. Mice in seven experimental groups were exposed to cigarette smoke, green tea, radiation or their mixtures respectively. One group was treated as the blank control group. The frequencies of micrnucleated polychromatic erythrocytes （MPCE） were scored by single blinded method. The data were analyzed with factorial experiment＇s analysis of variance using SAS 8,0. Results Analysis of variance showed that radiaton, cigarette smoke and green tea were independently significant factors （P 〈 0.01 ）. Interactions between cigarette smoke and radiation or between green tea and radiation were statistically significant （ P 〈 0.01 ）. Conclusion Radiaton and cigarette smoke can cause bone marrow cell mutations independently. There is synergistic effect between cigarette smoke and radiation. Green tea can inhibit radiation-induced cell mutation.