脆性组氨酸三聚体蛋白和蛋白激酶Cα的表达及其与非小细胞肺癌侵袭、转移的相关性

A study on the correlation between FHIT protein and cPKCα expression and invasion and metastasis in non-small cell lung cancer

目的探讨脆性组氨酸三聚体(FHIT)蛋白和蛋白激酶Cα(cPKCα)在非小细胞肺癌(NSCLC)中的表达及其与NSCLC侵袭、转移的相关性。方法采用免疫组化PicTureTM通用型二步法,对41例NSCLC组织和11例肺良性病变组织行FHIT蛋白和cPKCα检测。结果FHIT蛋白和cPKCα在NSCLC组织中的阳性表达率分别为48.8%和53.7%,在肺良性病变组织中的阳性表达率分别为90.9%和18.20%,均有显著性差异(P<0.05);COX比例风险模型生存分析结果显示,FHIT蛋白(P=0.020,RR=2.754)、cPKCα(P=0.022,RR=0.385)具有独立的预后意义。结论FHIT蛋白和cPKCα表达与NSCLC的发展、侵袭及转移显著相关,可作为判断NSCLC临床预后的参考指标。

Objective To investigate the role of FHIT protein and cPKCa in invasion and metastasis in non- small cell lung cancer （NSCLC）. Methods The expression of FHIT protein and cPKCa in 41 specimens of NSCLC and 11 cases benign lung tissues were examined by immunohistochemical technique. Results The positive rate of FHIT protein and cPKCa in NSCLC tissues were 48.8% and 53.7% respectively. The positive rate of FHIT protein and cPKCa in benign lung tissues were 90.9% and 18.2% respectively. They were significantly different （P〈0.05）. The results of the proportioned hazards regression model （Cox model） showed that FHIT protein （P= 0. 020, RR =2. 754）, cPKCa （ P=0. 022, RR =0. 385） were independent prognosis factors of NSCLC. Conclusion The expression of FHIT protein and cPKCa were closely related to the invasion and metastasis of NSCLC. The examination of FHIT protein and cPKCa would be independent factors which had correlation with prognosis of NSCLC.