大鼠肝细胞癌Ang2和Tie2表达与肿瘤血管生成的关系

Expression of Ang2 and Tie2 and their relation with the angiogenesis of hepatocellular carcinoma in rats

目的:探讨肝细胞癌中Ang2和Tie2表达与肿瘤血管生成的关系。方法:清洁级雄性Wis-tar大鼠38只,随机分为对照组5只、实验组25只(5,10,15,20,25d组各5只)及实验补充组8只,采用免疫抑制法建立同种移植大鼠CBRH-7919肝细胞癌模型;采用免疫组织化学染色方法检测不同生长阶段实验组肝癌组织和对照组肝组织标本中Ang2和Tie2的表达,同时测定抗CD31标记的微血管密度。结果:共24只大鼠成功建模。肝细胞癌组织中Ang2和Tie2的表达均较对照组肝组织明显增高(P<0.05)。Ang2和Tie2的表达水平均与肿瘤微血管密度呈正相关(P<0.01)。在不同生长阶段Ang2呈早期、持续性高表达。结论:肝细胞癌血管生成与Ang2和Tie2的表达上调有关,Ang2可能参与了肝细胞癌血管生成的启动环节。

Objective To investigate the relationship between the expression of Ang2, Tie2 and the angiogenesis of hepatocellular carcinoma in rats. Methods Thirty-eight healthy male rats were randomly divided into 3 groups： 5 rats in the control group; 25 rats in the experimental group were equally divided into 5-day, 10-day, 15-day, 20-day, and 25-day groups; the other 8 rats were used as the supplement of the experimental group. An allogenic transplanted rat model of CBRH-7919 hepatocellular carcinoma in situ was established by immunosuppression. The expressions of Ang2 and Tie2 were detected by immunohistochemical staining in cancerous tissues of different developmental stages and liver tissues of the control group. At the same time, microvessel density was determined by anti-CD31 immunohistochemical staining. Results CBRH-7919 hepatocellular carcinoma models were successfully set up in 24 rats. The expression level of Ang2 and Tie2 in cancerous tissues was much higher than that of liver tissues of the control group （ P 〈 0.05 ）. The overexpression of Ang2 was pristine and continuous in different developmental stages. The expressions of Ang2 and Tie2 positively correlated with microvessal density in hepatocellular carcinoma （ P 〈 0. 05 ）. Conclusion The up-regulation of Ang2 and Tie2 may play important roles in the angiogenesis of hepatocellular carcinoma. Ang2 may participate in the start of angiogenesis of hepatocellular carcinoma.