白细胞介素-1β基因多态性与儿童哮喘的相关性

Study on Association between Interleukin-1 Beta Gene Polymorphism and Childhood Asthma

目的探讨IL-1β基因第5外显子多态性与儿童哮喘的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法,检测55例哮喘患儿和35例健康儿童IL-1β基因第5外显子基因型和等位基因频率,比较两组IL-1β基因第5外显子的基因型和等位基因分布频率。结果哮喘组和健康组3种基因型CC、CT、TT分布频率分别为92.7%、7.3%、0;85.7%、14.3%、0,两组基因型分布差异无显著性(P>0.05)。哮喘组和健康组C和T等位基因分布频率分别为96.4%、3.6%;92.9%、7.1%,两组等位基因分布频率差异无显著性(P>0.05)。结论IL-1β基因第5外显子多态性可能与儿童哮喘易感性无关。

Objective To evaluae possible association between interleuldn - 1 beta （ IL - 1β） gene exon 5 polyrnorphism and childhood asthma. Methods The study was conducted in two different groups： asthmatic children（ n = 55） and healthy children（ n = 35）. The IL- 1β gene exon 5 polyrnorphism was genotyped by polymerase chain reaction - restriction fragment length polyrnorphism analysts （ PCR - RFLP）. Results Frequencies of CC,CT and TT genotypes were 92.7% ,7.3% ,0, and frequencies of C,T allele were 96.4%, 3.6% in asthmatic group. However, frequencies of CC,CA and AA genotypes were 85.7%, 14.3% ,0, and frequencies of C, T allele were 92.9 %, 7.1% in healthy group. There were no significant difference in distribution of genotypes and allele frequencies between two grouos. Conclusion IL - 1β gene exon 5 polymorphism may not be associated with childhood asthma.