绒毛膜促性腺激素对双侧隐睾大鼠睾丸生殖细胞凋亡的影响

Influence of Human Chorionic Gonadotropin on Testicular Germ Cell Apoptosis of Bilateral Cryptorchidism in Rats

目的探讨绒毛膜促性腺激素(HCG)对双侧隐睾生殖细胞凋亡的影响。方法SD雄性幼鼠于日龄22d制备双侧隐睾模型(模型组)后开始隔日肌注HCG20U,共7次。假手术组作为对照。日龄35、60d时处死取睾丸,采用生物素-dUTP/酶标亲和素(TUNEL)法检测生殖细胞凋亡情况。结果模型组双侧隐睾睾丸凋亡指数(AI)高于假手术组。35d假手术HCG治疗组AI高于假手术HCG未治疗组(P<0.05)。60d各HCG治疗组睾丸的AI高于相应HCG未治疗组,组间比较无显著差异(P>0.05)。结论隐睾时睾丸生殖细胞凋亡增加;HCG可增加生殖细胞的凋亡。

Objective To investigate the influence of human chorionic gonadotropin on testicular germ cell apoptosis of bilateral cryptorchidism in rats. Methods Immature male rats （22 day - old Sprague Dawley） were subjected to bilateral cryptorchidism and injected with 20 U human chorionic gonadotropin from the 22th day to the 34th day every other day. Sham operation rats were as control group. At age 35 days and 60 days, rats were sacrificed for detection germ cell apoptosis by terminal - deoxynucleotidyl transferase mediated d - UTP nick end labeling （TUNEL）. Results There were significant difference of apoptosis index （AI） between bilateral cryptorchidisxn and sham operation groups（ P〈 0.05 ）. There were significant difference of AI in sham operation group at age 35 days with and without HCG（P〈0.05）. Compared to correspond groups without HCG, AI of groups with HCG were higher at age 60 days,but there were no significant difference in every group（P 〉 0.05 ）. Conclusion AI of cryptorchidisxn increases and HCG addes the number of apoptotic germ cells.