益肾软坚煎抑制脂肪肝脂质过氧化的实验研究

Experimental Study on Inhibitory Effect of Yishenruanjianjian Decoction on Fatty Liver Lipid Peroxidation

目的观测益肾软坚煎治疗脂肪肝的疗效及其可能机制。方法采用高脂饮食造模,吉非罗齐为对照观察益肾软坚煎对脂肪肝大鼠肝组织匀浆中总胆固醇(TC)、甘油三脂(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)及转氨酶变化的影响,观察肝脏组织学改变。结果肝匀浆中TC,TG含量中药高剂量(H)组明显低于模型(M)组(P<0.05),H组肝匀浆中MDA含量明显低于M组,SOD活力高于M组(P<0.05);转氨酶水平H组接近于正常对照(N)组,无统计学差异(P>0.05)。结论益肾软坚煎有明显减少机体内脂质过氧化终产物,增加抗氧化能力的作用,且应用安全。

Objective its possible mechanism. Methods To study the effect of Yishenruanjianjian Rat NAFLD models were induced by high on nonalcoholic： fatty liver disease（NAFLD） and fat diet. C, emfibrozil was chosen to be the control medicine. The pathologieal changes of liver, levels of ALT, AST in serum, levels of TC, TG, SOD, MDA in liver were monitored. Results Contents of TC, TG were decreased highly in group H than that in group M （ P 〈 0.05 ） ; MDA was decreased and SOD activities were increased in group H than in group M （ P 〈 0.05） . Aminotransferase activities of group H were similar with group N. Conclusion Yishenruanjianjian not only decreases levels of liver lipid, lipid peroxide products but benefits the recovery of nonalcoholic fatty liver.