兔髁突软骨细胞与羊膜间生物相容性研究

A Study on the Biocompatibility Between Rabbit Mandibular Condylar Cells and Amnion

目的:研究羊膜作为颞下颌关节组织工程支架材料应用的的可行性。方法:采用Ⅱ型胶原酶消化解离兔髁突软骨中的纤维软骨细胞,取经TGF-β1扩增的第二代细胞与羊膜复合培养,进行MTT、光镜、扫描电镜观察。结果:髁突细胞于羊膜上牢固黏附,第3天开始增殖,3周时已有大量细胞生长于羊膜上。结论:羊膜表现出良好的吸水性、细胞吸附性和体外生物相容性,髁突细胞可在其上黏附、增殖,羊膜适于作为髁突组织工程支架材料。

Objective： To investigate the possibility of amnion as cell scaffold in tissue engineering of temporomandibular joint. Methods： Rabbit mandibular condylar fibrocartilage was digested by collagenase, and cells of the second generation proliferated by TGF - β1 were cultured onto amnion. Observation was made with MTT, light microscope, and electronic scanning microscopy. Results： Fibrocartilage cells attached to amnion closely and began to proliferate on the 3rd day. In 3 weeks after the transplantation, a lot of cells grew well on amnion. Conclusion： Amnion has good water adsorptivity and biocompatibility with fibrocartilage cells. It can be used as scaffold for tissue engineering of temporomandbular joint.