不同强度+Gz暴露对大鼠心肌细胞凋亡的影响

Effects of different +Gz exposure on cardiocyte apoptosis in rats

目的探讨不同强度的持续性正加速度(+Gz)对大鼠心肌细胞凋亡的影响.方法12只SD大鼠随机分为对照组、+6Gz暴露组、+10Gz暴露组,每组4只,+Gz暴露组分别给予+6Gz和+10Gz负荷刺激3min,于暴露后6h取大鼠心肌组织,用原位末端标记法(TUNEL)检测心肌细胞凋亡情况并计数.结果对照组大鼠偶见TUNEL阳性染色的心肌凋亡细胞;+6Gz暴露组大鼠心肌凋亡细胞数较对照组显著增多(P<0.05);+10Gz暴露组大鼠心肌凋亡阳性细胞数较对照组及+6Gz组均明显增多(P<0.05).结论+Gz暴露可以引起大鼠心肌细胞凋亡,且随着G值的增大,心肌凋亡细胞数目逐渐增多.

AIM： To explore the effects of different ＋ Gz exposure on cardiocyte apoptosis in rats. METHODS： Twelve rats were evenly randomized into 3 groups： control group, ＋ 6Gz/3 min group and ＋ 10Gz/3 min group. The rats were respectively killed 6 h after ＋ Gz exposure and the rate of cardiocyte apoptosis was counted after straining with terminal deoxynucleotide transferase-mediated dUTP nick end labeling （TUNEL） method. RESULTS： The cardiocyte apoptosis in control group was scarely observed, while it occurred numerously in the ＋ Gz exposure groups. Compared with the control group, the number of cardiocyte apoptosis increased significantly in ＋ 6Gz exposure group （ P 〈 0. 05 ）. The number of cardiocyte apoptosis increased significantly in the ＋ 10Gz/3 min group than that in the ＋ 6Gz/3 min group and control group （ P 〈 0. 05 ）. CONCLUSION： The cardiocyte apoptosis can be induced with ＋ Gz exposure in rats. With the increase of ＋ Gz values, the number of cardiocyte apoptosis increases gradually.