S100A4蛋白在非小细胞肺癌中的表达与侵袭和转移的关系

Correlations of S100A4 Protein Expression to Invasion and Metastasis of Non-small Cell Lung Cancer

背景与目的:实验证明S100A4在多种恶性肿瘤细胞中高表达,它可能在恶性肿瘤细胞的侵袭和转移等过程中发挥重要作用。本研究探讨S100A4在人非小细胞肺癌中的表达及其与侵袭和转移的关系。方法:采用免疫组化SP法检测41例非小细胞肺癌及6例正常肺组织中S100A4蛋白的表达水平。结果:S100A4在非小细胞肺癌中的阳性率(70.7%)显著高于正常肺组织(16.7%)(P<0.05);在肺腺癌中的阳性率(90.0%)明显高于肺鳞癌(52.4%)(P<0.01)。在TNM分期中,S100A4在Ⅲ+Ⅳ期、Ⅱ期和Ⅰ期的阳性率分别为100.0%、66.7%和30.0%,Ⅲ+Ⅳ期明显高于Ⅱ期和Ⅰ期(P<0.01),但是Ⅱ期和Ⅰ期间无显著性差异(P>0.05)。S100A4的阳性率在有淋巴结转移的非小细胞肺癌中(90.0%)明显高于无淋巴结转移组(52.4%)(P<0.01),且S100A4的表达与淋巴结转移密切相关(r=0.480,P=0.001)。肿瘤体积增大(<3cm,≥3cm),S100A4的阳性率明显升高(44.4%,91.3%)(P<0.001),且相关性显著(r=0.288,P=0.017)。S100A4的阳性率与非小细胞肺癌的病理分级无明显相关性(P>0.05)。结论:S100A4在非小细胞肺癌中的表达上调,且与淋巴结转移、TNM分期和肿瘤大小密切相关,提示S100A4与非小细胞肺癌的侵袭和转移有关。

BACKGROUND ＆ OBJECTIVE, Metastasis-associated protein S100A4 is overexpressed in many malignant tumor cells; it may play a pivotal role in invasion and metastasis of malignant tumors. This study was to determine the expression of S100A4 in human non-small cell lung cancer （NSCLC）, and to investigate its correlations to invasion and metastasis of NSCLC. METHODS. The expression of S100A4 in 41 specimens of NSCLC and 6 specimens of normal lung tissues was detected by SP immunohistochemistry. The correlations of S100A4 to clinicopathologic features of NSCLC were analyzed. RESULTS.. The positive rate of S100A4 was significantly higher in NSCLC than in normal lung tissues （70.7% vs. 16.7%, P〈0.05）, and was significantly higher in adenocarcinoma than in squamous cell carcinoma （90.0% vs. 52.4%, P〈0.01）. The positive rate of S100A4 was significantly higher in stage Ⅲ -Ⅳ than in stage Ⅱ and stage Ⅰ NSCLC （100.0% vs. 66.7% and 30.0%, P〈0.01）, while there was no obvious difference between the latter 2 groups （P〉0.05）. The positive rate of S100A4 was significantly higher in NSCLC with lymphatic metastasis than in NSCLC without lymphatic metastasis （90.0% vs. 52.4%, P〈0.01）, and significantly higher in NSCLC with tumor size of ≥ 3 cm than in NSCLC with tumor size of 〈 3 cm （91.3% vs. 44.4%, P〈0.001）. The expression of S100A4 was closely related to lymphatic metastasis （r=0.480, P=0.001）, and tumor size （r=0.288, P=0.017）. No significant correlation was found between the expression of S100A4 and pathologic grade of NSCLC （P 〉0.05）. CONCLUSION. S100A4 expression is up-regulated in NSCLC, and closely related to lymphatic metastasis, TNM stage and tumor size, which suggest an important role of S100A4 in the invasion and metastasis of NSCLC.