奥曲肽对系统性红斑狼疮患者体外培养单一核细胞增殖及分泌细胞因子的影响

Effect of octreotide on the secretion of cytokines by and proliferation of cultured PBMCs from patients with systemic lupus erythematosus

目的探讨奥曲肽对SLE患者体外培养单一核细胞增殖及其分泌细胞因子的影响。方法对SLE患者和正常人对照的单一核细胞进行体外培养,用MTT法测定单一核细胞加入不同浓度奥曲肽后的细胞增殖率,计算不同浓度奥曲肽对单一核细胞的抑制率,同时以生长激素加奥曲肽进行干预,并测定细胞因子的分泌水平。结果0．1 ng/mL奥曲肽对单一核细胞增殖无抑制作用,1 ng/mL奥曲肽开始有微弱的抑制作用,10 ng/mL奥曲肽有明显的抑制作用,且呈剂量依赖性。SLE患者外周血单一核细胞在体外经奥曲肽干预后,其IL-6、IL-10和IFN-γ分泌量较未处理组显著下降,生长激素加奥曲肽共同干预后的分泌量较生长激素干预的分泌量显著下降。结论奥曲肽体外能抑制单一核细胞增殖,且能抑制生长激素诱导的细胞因子的分泌。

Objective To investigate the effect of octreotide （ OCT ） on the proliferation capacity of and secretion of cytokines by cultured PBMCs from patients with systemic lupus erythematosus （ SLE ）. Methods PBMCs from patients with SLE and healthy controls were cultured in vitro with various concentrations of OCT, pretreated with or without growth hormone （ GH ）. The proliferation capacity of PBMCs was detected by MTT method, and the secretion of cytokines by PBMCs was measured with ELISA. Restilts OCT could inhibit the proliferation capacity of PBMCs in a dose dependent manner： 0.1 ng/mL of OCT had no effect on the proliferation, 1 ng/mL OCT had a weak inhibitory effect, and 10 ng/mL OCT had a marked inhibitory effect. The secretion of IL-6, IL-10 and IFN-γ by PBMCs was significantly decreased with the treatment of OCT, and was also significantly decreased with the co-treatment of GH and OCT compared to that with the treatment of GH only. Conclusion OCT can inhibit the proliferation capacity of PBMCs and secreation of IL-6, IL-10 and IFN-γ by PBMCs induced by GH.